caa a22 4500 475787560 CHVBK 20180406123703.0 cr unu---uuuuu 170329e20001201xx s 000 0 eng 10.1007/s004140000139 doi (NATIONALLICENCE)springer-10.1007/s004140000139 Forensic validation of a multiplex containing nine STRs - population genetics in Northern Poland [Elektronische Daten] [R. Pawlowski, A. Maciejewska] This paper presents the allele frequency distributions for the nine loci (D3S1358, VWA, FGA, D8S1179, D21S11, D18S51, D5S818, D13S317 and D7S820) present in the commercially available Profiler Plus kit. DNA samples of 202 individuals from Northern Poland were amplified in a multiplex reaction with subsequent automatic detection using capillary electrophoresis. All loci met the Hardy-Weinberg equilibrium conditions. The calculated probability of identity was 2.26 × 10-11 giving an average probability of identity of 1 in 44 billion. Considerable improvement of analysis precision was observed after substituting the GeneScan 500 for the fluorescent ladder 60-400 bp. The influence of DNA concentration on stutter and artefact formation and the ratio of heterozygote alleles was analysed. Springer-Verlag Berlin Heidelberg, 2000 Key words Profiler Plus nationallicence Validation studies nationallicence Capillary nationallicence electrophoresis nationallicence Population genetics nationallicence Poland nationallicence Pawlowski R. Institute of Forensic Medicine, Medical University of Gdansk, 80-210 Gdansk, Debinki 7, Poland e-mail: Richard@amg.gda.pl; Fax: +48-58-3410485, PL aut Maciejewska A. Institute of Forensic Medicine, Medical University of Gdansk, 80-210 Gdansk, Debinki 7, Poland e-mail: Richard@amg.gda.pl; Fax: +48-58-3410485, PL aut https://doi.org/10.1007/s004140000139 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/s004140000139 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Pawlowski R. Institute of Forensic Medicine, Medical University of Gdansk, 80-210 Gdansk, Debinki 7, Poland e-mail: Richard@amg.gda.pl; Fax: +48-58-3410485, PL aut NATIONALLICENCE 700 1- Maciejewska A. Institute of Forensic Medicine, Medical University of Gdansk, 80-210 Gdansk, Debinki 7, Poland e-mail: Richard@amg.gda.pl; Fax: +48-58-3410485, PL aut Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer