caa a22 4500 475787668 CHVBK 20180406123703.0 cr unu---uuuuu 170329e20000501xx s 000 0 eng 10.1007/s004140050285 doi (NATIONALLICENCE)springer-10.1007/s004140050285 The expression of mRNA of proinflammatory cytokines during skin wound healing in mice: a preliminary study for forensic wound age estimation (II) [Elektronische Daten] [Y. Sato, T. Ohshima] This study examined the temporal expression of mRNA for the proinflammatory cytokines, interleukin (IL)-1α, IL-1β, IL-6 and tumor necrosis factor-α (TNFα) in incised wounds in mice using the reverse transcriptase-polymerase chain reaction (RT-PCR) and in situ hybridization (ISH) techniques. After incision, an increase in each cytokine mRNA level was observed by RT-PCR. The local IL-6 mRNA level peaked at 6 h, while the peak levels of mRNA for IL-1α, IL-1β and TNFα occurred between 48 and 72 h. All cytokine mRNA levels were almost normalized after 240 h. In the early phase of wound healing, infiltrating polymorphonuclear cells were labeled with antisense probes for IL-1α, IL-1β and TNFα mRNA by the ISH technique. Thereafter, infiltrating mononuclear cells and spindle-shaped mesenchymal cells showed positive signals for all the cytokines examined. Regenerating epidermal cells were also labeled with the antisense probes for IL-1α, IL-6 and TNFα mRNA, indicating that IL-1, IL-6, and TNFα are involved in skin wound healing and their local production by various cells involved in the healing process is suggested. From the viewpoint of forensic pathology, the temporal characteristics of the cytokine mRNA expression may have a potential to indicate wound age or wound vitality. Springer-Verlag Berlin Heidelberg, 2000 Key words Wound healing nationallicence Wound age estimation nationallicence Skin nationallicence Proinflammatory cytokine nationallicence Messenger RNA nationallicence Sato Y. Department of Legal Medicine, Kanazawa University Faculty of Medicine, School of Medicine, Takara-machi 13-1, Kanazawa 920-8640, Japan Tel. +81-76-265-2222, Fax +81-76-234-4234, JP aut Ohshima T. Department of Legal Medicine, Kanazawa University Faculty of Medicine, School of Medicine, Takara-machi 13-1, Kanazawa 920-8640, Japan Tel. +81-76-265-2222, Fax +81-76-234-4234, JP aut https://doi.org/10.1007/s004140050285 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/s004140050285 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Sato Y. Department of Legal Medicine, Kanazawa University Faculty of Medicine, School of Medicine, Takara-machi 13-1, Kanazawa 920-8640, Japan Tel. +81-76-265-2222, Fax +81-76-234-4234, JP aut NATIONALLICENCE 700 1- Ohshima T. Department of Legal Medicine, Kanazawa University Faculty of Medicine, School of Medicine, Takara-machi 13-1, Kanazawa 920-8640, Japan Tel. +81-76-265-2222, Fax +81-76-234-4234, JP aut Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer