caa a22 4500 475787714 CHVBK 20180406123703.0 cr unu---uuuuu 170329e20000501xx s 000 0 eng 10.1007/s004140050296 doi (NATIONALLICENCE)springer-10.1007/s004140050296 Y-chromosomal STR haplotypes in a population sample from Bavaria [Elektronische Daten] [K. Anslinger, W. Keil, G. Weichhold, W. Eisenmenger] The seven Y-chromosomal STRs DYS19, DYS385 I/II, DYS389 I/II, DYS390, DYS391, DYS392 and DYS393 were amplified using two multiplex PCRs. The optimization of the PCR conditions led to reliable and sensitive systems. Co-amplification of the amelogenin locus was possible in both multiplex systems. In a population sample of 151 Bavarian males, a gene diversity of 0.99 was observed. Sensitivity studies revealed a detection limit of 50 pg DNA per 25 μl reaction volume. PCR experiments with combinations of male/male and male/female DNA showed that in male/male mixtures, the minor component could be detected up to a ratio of 1:15, whereas in male/female mixtures the male component could be found in a higher ratio up to 1: 60. Springer-Verlag Berlin Heidelberg, 2000 Key words Y-chromosome nationallicence STRs nationallicence Multiplex nationallicence Y haplotype nationallicence Mixtures nationallicence Anslinger K. Institute of Legal Medicine, Ludwig-Maximilians-University of Munich, Frauenlobstrasse 7 a, D-80337 Munich, Germany Tel. +49-89-51605121, Fax +49-89-51605144, DE aut Keil W. Institute of Legal Medicine, Ludwig-Maximilians-University of Munich, Frauenlobstrasse 7 a, D-80337 Munich, Germany Tel. +49-89-51605121, Fax +49-89-51605144, DE aut Weichhold G. Institute of Legal Medicine, Ludwig-Maximilians-University of Munich, Frauenlobstrasse 7 a, D-80337 Munich, Germany Tel. +49-89-51605121, Fax +49-89-51605144, DE aut Eisenmenger W. Institute of Legal Medicine, Ludwig-Maximilians-University of Munich, Frauenlobstrasse 7 a, D-80337 Munich, Germany Tel. +49-89-51605121, Fax +49-89-51605144, DE aut https://doi.org/10.1007/s004140050296 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/s004140050296 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Anslinger K. Institute of Legal Medicine, Ludwig-Maximilians-University of Munich, Frauenlobstrasse 7 a, D-80337 Munich, Germany Tel. +49-89-51605121, Fax +49-89-51605144, DE aut NATIONALLICENCE 700 1- Keil W. Institute of Legal Medicine, Ludwig-Maximilians-University of Munich, Frauenlobstrasse 7 a, D-80337 Munich, Germany Tel. +49-89-51605121, Fax +49-89-51605144, DE aut NATIONALLICENCE 700 1- Weichhold G. Institute of Legal Medicine, Ludwig-Maximilians-University of Munich, Frauenlobstrasse 7 a, D-80337 Munich, Germany Tel. +49-89-51605121, Fax +49-89-51605144, DE aut NATIONALLICENCE 700 1- Eisenmenger W. Institute of Legal Medicine, Ludwig-Maximilians-University of Munich, Frauenlobstrasse 7 a, D-80337 Munich, Germany Tel. +49-89-51605121, Fax +49-89-51605144, DE aut Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer