caa a22 4500 47578779X CHVBK 20180406123704.0 cr unu---uuuuu 170329e20000601xx s 000 0 eng 10.1007/s004149900088 doi (NATIONALLICENCE)springer-10.1007/s004149900088 Methamphetamine induces an increase in cell size and reorganization of myofibrils in cultured adult rat cardiomyocytes [Elektronische Daten] [Y. Maeno, M. Iwasa, H. Inoue, H. Koyama, R. Matoba] To investigate the direct effects of methamphetamine (MAP) on cardiac lesions seen in MAP abusers, isolated adult rat ventricular cardiomyocytes (ARCs) were exposed to MAP (0.05-1.0 mM) in medium 199 containing 10% fetal calf serum. Isolated ARCs attached to laminin-coated substrata and began to spread into polygonal shapes with pseudopodia at day 6 in normal culture. However, the cell attachment and spreading were inhibited by exposure to MAP (0.5 and 1.0 mM) for the first 7 days in culture. On the other hand, exposure to MAP (0.05 and 0.1 mM) for 7 days after a 6-day period of normal culture, led to a larger cross surface area of cells with more abundant actin bundles compared to control cells (p < 0.05). This development of spreading area resembled that of norepinephrine-treated ARCs. In addition, immunoreactive atrial natriuretic peptide (ANP) granules developed and accumulated around the nuclear region of ARCs exposed to MAP and the number of ANP positive cells tended to increase in a dose-dependent manner. These results suggest that chronic exposure to a high concentration of MAP may directly inhibit development of ARCs in culture and that a continuous exposure to a low concentration of MAP may facilitate the development of cellular hypertrophy. Therefore, hypertrophied cardiomyocytes in MAP abusers may be provoked by multifactorial incidents of direct and indirect actions of MAP. Springer-Verlag Berlin Heidelberg, 2000 Key words Methamphetamine nationallicence Cardiotoxicity nationallicence Cardiomyocyte nationallicence Cell culture nationallicence Hypertrophy nationallicence Maeno Y. Department of Legal Medicine, Nagoya City University Medical School, Kawasumi 1, Mizuho-ku, Nagoya 467-8601, Japan e-mail: maenoyo@med.nagoya-cu.ac.jp, Tel.: +81-52-8538181, Fax: +81-52-8427701, JP aut Iwasa M. Department of Legal Medicine, Nagoya City University Medical School, Kawasumi 1, Mizuho-ku, Nagoya 467-8601, Japan e-mail: maenoyo@med.nagoya-cu.ac.jp, Tel.: +81-52-8538181, Fax: +81-52-8427701, JP aut Inoue H. Department of Legal Medicine, Nagoya City University Medical School, Kawasumi 1, Mizuho-ku, Nagoya 467-8601, Japan e-mail: maenoyo@med.nagoya-cu.ac.jp, Tel.: +81-52-8538181, Fax: +81-52-8427701, JP aut Koyama H. Department of Legal Medicine, Nagoya City University Medical School, Kawasumi 1, Mizuho-ku, Nagoya 467-8601, Japan e-mail: maenoyo@med.nagoya-cu.ac.jp, Tel.: +81-52-8538181, Fax: +81-52-8427701, JP aut Matoba R. Department of Legal Medicine, Osaka University Medical School, Yamadaoka 2-2, Suita, Osaka 565-0871, Japan, JP aut https://doi.org/10.1007/s004149900088 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/s004149900088 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Maeno Y. Department of Legal Medicine, Nagoya City University Medical School, Kawasumi 1, Mizuho-ku, Nagoya 467-8601, Japan e-mail: maenoyo@med.nagoya-cu.ac.jp, Tel.: +81-52-8538181, Fax: +81-52-8427701, JP aut NATIONALLICENCE 700 1- Iwasa M. Department of Legal Medicine, Nagoya City University Medical School, Kawasumi 1, Mizuho-ku, Nagoya 467-8601, Japan e-mail: maenoyo@med.nagoya-cu.ac.jp, Tel.: +81-52-8538181, Fax: +81-52-8427701, JP aut NATIONALLICENCE 700 1- Inoue H. Department of Legal Medicine, Nagoya City University Medical School, Kawasumi 1, Mizuho-ku, Nagoya 467-8601, Japan e-mail: maenoyo@med.nagoya-cu.ac.jp, Tel.: +81-52-8538181, Fax: +81-52-8427701, JP aut NATIONALLICENCE 700 1- Koyama H. Department of Legal Medicine, Nagoya City University Medical School, Kawasumi 1, Mizuho-ku, Nagoya 467-8601, Japan e-mail: maenoyo@med.nagoya-cu.ac.jp, Tel.: +81-52-8538181, Fax: +81-52-8427701, JP aut NATIONALLICENCE 700 1- Matoba R. Department of Legal Medicine, Osaka University Medical School, Yamadaoka 2-2, Suita, Osaka 565-0871, Japan, JP aut Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer