caa a22 4500 47578782X CHVBK 20180406123704.0 cr unu---uuuuu 170329e20000601xx s 000 0 eng 10.1007/s004149900098 doi (NATIONALLICENCE)springer-10.1007/s004149900098 Automated solid-phase extraction and two-step derivatisation for simultaneous analysis of basic illicit drugs in serum by GC/MS [Elektronische Daten] [W. Weinmann, M. Renz, S. Vogt, S. Pollak] A combination of automated solid-phase extraction (SPE) and subsequent two-step derivatisation has been developed for the simultaneous analysis of basic drugs of abuse and cocaine metabolites in serum samples. Substances included in this procedure are morphine, codeine, methadone, cocaine, benzoylecgonine, methylecgonine, amphetamine, methamphetamine, MDMA, MDEA and MDA. SPE with mixed-mode cartridges (RP-C8 and cation-exchange) was fully automated with a Zymark RapidTrace SPE robot. GC/MS analysis was performed after derivatisation with a new two-step reaction by trifluoroacetic anhydride and 2,2,3,3,3-pentafluoropropanol. High recoveries (> 85%) with high reproducibility ¶(CV 1.1-3.8%) were found for all drugs. High correlation coefficients (r > 0.998) were obtained due to the addition of deuterated standards prior to extraction. Experience obtained over 2 years of applying this method to drug analysis in serum is discussed. Springer-Verlag Berlin Heidelberg, 2000 Key words Drugs of abuse nationallicence Quantitative analysis nationallicence Automation nationallicence Solid-phase extraction nationallicence GC/MS nationallicence Derivatisation nationallicence Weinmann W. Institut für Rechtsmedizin, Klinikum der Albert-Ludwigs-Universität, Albertstrasse 9, D-79104 Freiburg, Germany e-mail: weinmann@sun11.ukl.uni-freiburg.de, Tel.: +49-761-2036856, Fax: +49-761-2036858, DE aut Renz M. Institut für Rechtsmedizin, Klinikum der Albert-Ludwigs-Universität, Albertstrasse 9, D-79104 Freiburg, Germany e-mail: weinmann@sun11.ukl.uni-freiburg.de, Tel.: +49-761-2036856, Fax: +49-761-2036858, DE aut Vogt S. Institut für Rechtsmedizin, Klinikum der Albert-Ludwigs-Universität, Albertstrasse 9, D-79104 Freiburg, Germany e-mail: weinmann@sun11.ukl.uni-freiburg.de, Tel.: +49-761-2036856, Fax: +49-761-2036858, DE aut Pollak S. Institut für Rechtsmedizin, Klinikum der Albert-Ludwigs-Universität, Albertstrasse 9, D-79104 Freiburg, Germany e-mail: weinmann@sun11.ukl.uni-freiburg.de, Tel.: +49-761-2036856, Fax: +49-761-2036858, DE aut https://doi.org/10.1007/s004149900098 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/s004149900098 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Weinmann W. Institut für Rechtsmedizin, Klinikum der Albert-Ludwigs-Universität, Albertstrasse 9, D-79104 Freiburg, Germany e-mail: weinmann@sun11.ukl.uni-freiburg.de, Tel.: +49-761-2036856, Fax: +49-761-2036858, DE aut NATIONALLICENCE 700 1- Renz M. Institut für Rechtsmedizin, Klinikum der Albert-Ludwigs-Universität, Albertstrasse 9, D-79104 Freiburg, Germany e-mail: weinmann@sun11.ukl.uni-freiburg.de, Tel.: +49-761-2036856, Fax: +49-761-2036858, DE aut NATIONALLICENCE 700 1- Vogt S. Institut für Rechtsmedizin, Klinikum der Albert-Ludwigs-Universität, Albertstrasse 9, D-79104 Freiburg, Germany e-mail: weinmann@sun11.ukl.uni-freiburg.de, Tel.: +49-761-2036856, Fax: +49-761-2036858, DE aut NATIONALLICENCE 700 1- Pollak S. Institut für Rechtsmedizin, Klinikum der Albert-Ludwigs-Universität, Albertstrasse 9, D-79104 Freiburg, Germany e-mail: weinmann@sun11.ukl.uni-freiburg.de, Tel.: +49-761-2036856, Fax: +49-761-2036858, DE aut Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer