caa a22 4500 475787900 CHVBK 20180406123704.0 cr unu---uuuuu 170329e20000601xx s 000 0 eng 10.1007/s004149900108 doi (NATIONALLICENCE)springer-10.1007/s004149900108 AgNORs during the process of wound healing [Elektronische Daten] Time dependency as evaluated in vital and postmortem biopsy [M. Rebolledo Godoy, A. P. Rebolledo Godoy, M. Oehmichen] Silver staining of paraffin sections to detect changes in nucleolar organizer regions (AgNORs) is an established method for detecting cellular proliferation. To determine whether AgNORs are helpful in assessing wound age and vitality, we examined intravital and postmortem skin biopsies from rats surviving incised wounds to both pinnae for defined intervals up to 384 h using, 7 rats per time interval. One biopsy was taken immediately before death, a second 24 h after been sacrificed and storage at 8 °C. Interactive computer-assisted image analysis revealed that in the first 120 h after trauma the total number of AgNORs, the mean AgNOR sum area in the nucleus, and the mean AgNOR area per nucleus were dependent on survival time. Taken as indicators of proliferative activity these morphological phenomena revealed an increase in proliferative activity after survival times ranging from 10 to a maximum of 96 h. The findings were the same in both intravital and postmortem biopsies. These findings are in accordance with those obtained using bromodeoxyuridine. The value of these experimental findings in assessing wound age of human beings is discussed. Springer-Verlag Berlin Heidelberg, 2000 Key words AgNOR nationallicence Proliferation nationallicence Wound nationallicence Intravital nationallicence Postmortem nationallicence Rebolledo Godoy M. Department of Legal Medicine, University of Lübeck, Kahlhorststrasse 31-35, 23562 Lübeck, Germany Fax: +49-451-500 2760 e-mail: oehmich@rmed.mu-luebeck.de, DE aut Rebolledo Godoy A. P. Department of Legal Medicine, University of Lübeck, Kahlhorststrasse 31-35, 23562 Lübeck, Germany Fax: +49-451-500 2760 e-mail: oehmich@rmed.mu-luebeck.de, DE aut Oehmichen M. Department of Legal Medicine, University of Lübeck, Kahlhorststrasse 31-35, 23562 Lübeck, Germany Fax: +49-451-500 2760 e-mail: oehmich@rmed.mu-luebeck.de, DE aut https://doi.org/10.1007/s004149900108 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/s004149900108 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Rebolledo Godoy M. Department of Legal Medicine, University of Lübeck, Kahlhorststrasse 31-35, 23562 Lübeck, Germany Fax: +49-451-500 2760 e-mail: oehmich@rmed.mu-luebeck.de, DE aut NATIONALLICENCE 700 1- Rebolledo Godoy A. P. Department of Legal Medicine, University of Lübeck, Kahlhorststrasse 31-35, 23562 Lübeck, Germany Fax: +49-451-500 2760 e-mail: oehmich@rmed.mu-luebeck.de, DE aut NATIONALLICENCE 700 1- Oehmichen M. Department of Legal Medicine, University of Lübeck, Kahlhorststrasse 31-35, 23562 Lübeck, Germany Fax: +49-451-500 2760 e-mail: oehmich@rmed.mu-luebeck.de, DE aut Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer