caa a22 4500 475788060 CHVBK 20180406123704.0 cr unu---uuuuu 170329e20000201xx s 000 0 eng 10.1007/s004140050011 doi (NATIONALLICENCE)springer-10.1007/s004140050011 Influence of different staining techniques on the DNA analysis of histological sections [Elektronische Daten] [S. Banaschak, B. Rolf, B. Brinkmann] Abstract : The use of stained histological sections as a source for DNA may be necessary in forensic case work if confusion of tissue is suspected, for identification or paternity cases. To elucidate the influence of different staining techniques on the PCR amplification of DNA, histological sections of liver tissue were prepared using eight different staining techniques and two histochemical methods. The DNA of the sections was extracted by a modified Chelex extraction and amplified using a commercial triplex kit. Staining with hematoxylin-eosin, hemalum-eosin, azan, periodic acid-schiff and prussian blue showed no adverse effect on the amplification of DNA while the extracts of tissue stained by Masson-Goldner, Ladewig and elastica-van Gieson methods had to be purified before amplification was possible. Staining with phosphoric tungsten acid hematoxylin and Gomori led to a degradation of DNA probably due to the use of potassium permanganate solution. Springer-Verlag Berlin Heidelberg, 2000 Key words Histological sections nationallicence Staining technique nationallicence DNA identification nationallicence Banaschak S. Institute of Legal Medicine, Westfälische Wilhelms-Universität, Von-Esmarch-Straße 62, D-48149 Münster, Germany Fax +49-251-8355158, DE aut Rolf B. Institute of Legal Medicine, Westfälische Wilhelms-Universität, Von-Esmarch-Straße 62, D-48149 Münster, Germany Fax +49-251-8355158, DE aut Brinkmann B. Institute of Legal Medicine, Westfälische Wilhelms-Universität, Von-Esmarch-Straße 62, D-48149 Münster, Germany Fax +49-251-8355158, DE aut https://doi.org/10.1007/s004140050011 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/s004140050011 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Banaschak S. Institute of Legal Medicine, Westfälische Wilhelms-Universität, Von-Esmarch-Straße 62, D-48149 Münster, Germany Fax +49-251-8355158, DE aut NATIONALLICENCE 700 1- Rolf B. Institute of Legal Medicine, Westfälische Wilhelms-Universität, Von-Esmarch-Straße 62, D-48149 Münster, Germany Fax +49-251-8355158, DE aut NATIONALLICENCE 700 1- Brinkmann B. Institute of Legal Medicine, Westfälische Wilhelms-Universität, Von-Esmarch-Straße 62, D-48149 Münster, Germany Fax +49-251-8355158, DE aut Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer