caa a22 4500 475790499 CHVBK 20180406123709.0 cr unu---uuuuu 170329e20000101xx s 000 0 eng 10.1007/s004230050010 doi (NATIONALLICENCE)springer-10.1007/s004230050010 Acceleration of wound healing by topical drug delivery via liposomes [Elektronische Daten] [Frank Roesken, Eberhard Uhl, Sergio B. Curri, Michael D. Menger, Konrad Messmer] Abstract : Background: Despite intensive research, impaired wound healing remains a considerable complication. Therefore, topically applied liposome-encapsulated buflomedil hydrochloride was investigated for its ability to improve wound repair in normal (n=16) and ischemic (n=16) skin tissue. Methods: Experiments were performed using the wound healing model of the ear of hairless mice. Standardized skin wounds (4.25 mm2) were created by circular excision of the epidermal layer and the subcutaneous tissue. Liposomes were applied daily until complete neovascularization of the wound occurred. Tissue regeneration by complete epithelialization and neovascularization of the wound area, microcirculatory parameters, and leukocyte-endothelium interaction were investigated by means of intravital microscopy. Microvascular perfusion was assessed by laser-Doppler flowmetry. Results: Topical application of buflomedil liposomes led to a significantly (P<0.05) accelerated wound closure in both normal (9.6±0.7 days) and ischemic (13.4±0.1 days) skin tissue compared with animals that were treated with unloaded liposomes (controls; 13.1±0.8 days; 15.3±0.6 days). Complete neovascularization of the wound was also enhanced (P<0.05) in buflomedil-treated animals (normal tissue 18.8±0.4 days; ischemic tissue 19.6±0.7 days) compared with controls (20.6±0.6 days; 22.6±1.2 days). Conclusion: These data suggest that buflomedil-loaded liposomes might be of beneficial use for clinical wound care. Springer-Verlag Berlin Heidelberg, 2000 Key words Topical drug application nationallicence Liposomes nationallicence Buflomedil hydrochloride nationallicence Wound healing nationallicence Epithelialization nationallicence Neovascularization nationallicence Hairless mouse nationallicence Intravital fluorescence microscopy nationallicence Roesken Frank Institute for Surgical Research, Ludwig-Maximilians-University, Munich, Germany, DE aut Uhl Eberhard Institute for Surgical Research, Ludwig-Maximilians-University, Munich, Germany, DE aut Curri Sergio B. Centro di Biologica Molecolare, Milano, Italy, IT aut Menger Michael D. Institute for Clinical and Experimental Surgery, University of Saarland, Homburg/Saar, Germany, DE aut Messmer Konrad Institute for Surgical Research, Ludwig-Maximilians-University, Munich, Germany, DE aut https://doi.org/10.1007/s004230050010 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/s004230050010 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Roesken Frank Institute for Surgical Research, Ludwig-Maximilians-University, Munich, Germany, DE aut NATIONALLICENCE 700 1- Uhl Eberhard Institute for Surgical Research, Ludwig-Maximilians-University, Munich, Germany, DE aut NATIONALLICENCE 700 1- Curri Sergio B. Centro di Biologica Molecolare, Milano, Italy, IT aut NATIONALLICENCE 700 1- Menger Michael D. Institute for Clinical and Experimental Surgery, University of Saarland, Homburg/Saar, Germany, DE aut NATIONALLICENCE 700 1- Messmer Konrad Institute for Surgical Research, Ludwig-Maximilians-University, Munich, Germany, DE aut Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer