caa a22 4500 475790677 CHVBK 20180406123709.0 cr unu---uuuuu 170329e20001101xx s 000 0 eng 10.1007/s004230000174 doi (NATIONALLICENCE)springer-10.1007/s004230000174 Fluorescence detection of small gastrointestinal tumours: principles, technique, first clinical experience [Elektronische Daten] [K. Orth, D. Russ, R. Steiner, H.G. Beger] Abstract.: Photodynamic therapy (PDT) is a form of cancer treatment based on the selective accumulation of a photosensitizer in neoplastic tissue. The fluorescent properties of a photosensitizer permit diagnostic localization of primary tumours and/or metastasis. Occult lesions are hard to detect and can easily be missed during routine laparoscopy. Fluorescence observation offers additional optical information and the ability to detect these occult tumours. Clinically, we used 5-aminolevulinic acid for peritoneal staining and tumour demarcation via tumour-specific fluorescence induced by protoporphyrin IX. For laparoscopic observations, a "D-Light" system was used; the conventional white light source was equipped with an optical blocking filter that transmits at the excitation wavelength (380-450nm) and blocks all other parts of the spectrum. With the aid of a suitable observation filter, the relevant fluorescence was detectable. With the help of this fluorescence we increased the capacity to detect occult tumours, that were missed with white-light observation (9/26). In the gastrointestinal tract, we used a krypton laser at 405nm for PP IX fluorescence induction. Although there were high sensitivity rates for neoplasms (81% peritoneal carcinomas, 60% gastric cancer), no exact histopathological statement could be achieved at because of false-positive fluorescence, mainly caused by inflammation (6/32). Current clinical goals and the future perspectives of photodynamic diagnostic are discussed. Springer-Verlag, 2000 Fluorescence tumour detection 5-Aminolevulinic acid nationallicence Orth K. Department of General Surgery, University of Ulm, Steinhövelstraße 9, 89075 Ulm, Germany aut Russ D. Institute for Laser Technology in Medicine, Ulm, Germany aut Steiner R. Institute for Laser Technology in Medicine, Ulm, Germany aut Beger H.G. Department of General Surgery, University of Ulm, Steinhövelstraße 9, 89075 Ulm, Germany aut https://doi.org/10.1007/s004230000174 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/s004230000174 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Orth K. Department of General Surgery, University of Ulm, Steinhövelstraße 9, 89075 Ulm, Germany aut NATIONALLICENCE 700 1- Russ D. Institute for Laser Technology in Medicine, Ulm, Germany aut NATIONALLICENCE 700 1- Steiner R. Institute for Laser Technology in Medicine, Ulm, Germany aut NATIONALLICENCE 700 1- Beger H.G. Department of General Surgery, University of Ulm, Steinhövelstraße 9, 89075 Ulm, Germany aut Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer