caa a22 4500 475791045 CHVBK 20180406123710.0 cr unu---uuuuu 170329e20001201xx s 000 0 eng 10.1007/s004230000168 doi (NATIONALLICENCE)springer-10.1007/s004230000168 Postischemic cardiac function recovery in the isolated rat heart: effects of adenosine deaminase and nucleoside transport inhibition [Elektronische Daten] [Ingeborg M. Keeling, Rudolf P. Obermayr, Barbara Schneider, Paul G. Spieckermann] Abstract.: Background and aims: This study assessed the cardioprotective effects of inhibitors of adenosine metabolism in an isolated perfused rat heart model. Specifically, we studied the adenosine deaminase inhibitor erythro-9-(2-hydroxy-3-nonyl)-adenine and the selective nucleoside transport inhibitor S-(p-nitrobenzyl)-6-thioinosine, in terms of their potential to enhance protection when added to Bretschneider's cardioplegic solution. Methods: Rat hearts were infused for 5min with Krebs-Henseleit buffer solution (group 1), Bretschneider's cardioplegic solution (group 2), Bretschneider's cardioplegic solution with the addition of 25µM erythro-9-(2-hydroxy-3-nonyl)-adenine and 5µM S-(p-nitrobenzyl)-6-thioinosine (group 3), and Bretschneider's cardioplegic solution with the addtion of 25µM erythro-9-(2-hydroxy-3-nonyl)-adenine only (group 4). After cardioplegic arrest and 45min of ischemic storage at 25°C, the functional recovery of the hearts was tested during 15min of Langendorff reperfusion and then 45min of working heart reperfusion. Results: In relation to the cardioprotective effects of Bretschneider's cardioplegic solution alone, we observed an improved recovery of hemodynamic function of the hearts with the addition of both erythro-9-(2-hydroxy-3-nonyl)-adenine and S-(p-nitrobenzyl)-6-thioinosine. However, the myocardial adenosine triphosphate (ATP) concentration remained unchanged. Bradycardia observed under the addition of erythro-9-(2-hydroxy-3-nonyl)-adenine alone was prevented by the addition of S-(p-nitrobenzyl)-6-thioinosine. Conclusion: A combination of both substances may be tested further for cardiac preservation, as it might improve the recovery from ischemia at moderate temperatures. Springer-Verlag, 2000 Erythro-9-(2-hydroxy-3-nonyl)-adenine (EHNA) S-(p-nitrobenzyl)-6-thioinosine (NBMPR) Bretschneider's cardioplegic solution Ischemia-reperfusion injury Working-heart model for rats nationallicence Keeling Ingeborg M. Department of Surgery, Division for Cardiac Surgery, University Hospital, Auenbruggerplatz 1, 8010 Graz, Austria aut Obermayr Rudolf P. Institute of Medical Physiology, University of Vienna, Austria aut Schneider Barbara Institute of Medical Statistics, University of Vienna, Austria aut Spieckermann Paul G. Institute of Medical Physiology, University of Vienna, Austria aut https://doi.org/10.1007/s004230000168 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/s004230000168 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Keeling Ingeborg M. Department of Surgery, Division for Cardiac Surgery, University Hospital, Auenbruggerplatz 1, 8010 Graz, Austria aut NATIONALLICENCE 700 1- Obermayr Rudolf P. Institute of Medical Physiology, University of Vienna, Austria aut NATIONALLICENCE 700 1- Schneider Barbara Institute of Medical Statistics, University of Vienna, Austria aut NATIONALLICENCE 700 1- Spieckermann Paul G. Institute of Medical Physiology, University of Vienna, Austria aut Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer