caa a22 4500 475791142 CHVBK 20180406123711.0 cr unu---uuuuu 170329e20000801xx s 000 0 eng 10.1007/s004230000154 doi (NATIONALLICENCE)springer-10.1007/s004230000154 Differential effects of neurotensin and cholecystokinin on intestinal microcirculation after ischemia-reperfusion [Elektronische Daten] [M. Heuser, O. Gralla, O. Pfaar, R. Nustede, H.J. Gröne, S. Post] Abstract.: Background: We investigated the effect of neurotensin and cholecystokinin (CCK) on intestinal microcirculation after ischemia-reperfusion. Method: Ischemia was induced in Wistar rats by occlusion of the superior mesenteric artery for 40min. Ten minutes before reperfusion, infusion of either neurotensin or CCK was started. Afterwards, the microhemodynamics of the jejunum were examined by means of intravital microscopy. Results: Ischemia-reperfusion decreased functional capillary density from 873.4±18.1 to 362.5±8.3cm-1 and red blood cell velocity from 0.49±0.03 to 0.34±0.02mm/s. Furthermore, leukocyte-endothelium interaction was increased. Neurotensin infusion significantly increased functional capillary density to 483.2±9.0cm-1 and red blood cell velocity to 0.69±0.01mm/s in the mucosal capillaries compared with ischemic controls. Despite the amelioration of villus perfusion, the number of non-perfused villi significantly increased (11.8±3.6%) compared with ischemic controls. CCK infusion also resulted in a significant increase of functional capillary density (535.2±7.4cm-1) and red blood cell velocity (0.67±0.01mm/s). In contrast to neurotensin, the number of non-perfused villi was not increased (5.8±2.2%). Conclusion: We conclude that neurotensin further aggravates perfusion inhomogeneity and stasis when administered during the ischemic period. In contrast, CCK has no negative influence on perfusion homogeneity after ischemia-reperfusion. It may be superior to neurotensin in the reconstitution of normal microvascular perfusion patterns after ischemia-reperfusion. Springer-Verlag, 2000 Intestine Intravital microscopy Wistar rat Intestinal steal phenomenon Neurotensin Cholecystokinin nationallicence Heuser M. Department of General Surgery, Georg-August-University, Göttingen, Germany aut Gralla O. Department of General Surgery, Georg-August-University, Göttingen, Germany aut Pfaar O. Department of General Surgery, Georg-August-University, Göttingen, Germany aut Nustede R. Department of General Surgery, Georg-August-University, Göttingen, Germany aut Gröne H.J. Department of Pathology, Philipps-University, Marburg, Germany aut Post S. Department of General Surgery, Georg-August-University, Göttingen, Germany aut https://doi.org/10.1007/s004230000154 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/s004230000154 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Heuser M. Department of General Surgery, Georg-August-University, Göttingen, Germany aut NATIONALLICENCE 700 1- Gralla O. Department of General Surgery, Georg-August-University, Göttingen, Germany aut NATIONALLICENCE 700 1- Pfaar O. Department of General Surgery, Georg-August-University, Göttingen, Germany aut NATIONALLICENCE 700 1- Nustede R. Department of General Surgery, Georg-August-University, Göttingen, Germany aut NATIONALLICENCE 700 1- Gröne H.J. Department of Pathology, Philipps-University, Marburg, Germany aut NATIONALLICENCE 700 1- Post S. Department of General Surgery, Georg-August-University, Göttingen, Germany aut Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer