caa a22 4500 475791215 CHVBK 20180406123711.0 cr unu---uuuuu 170329e20000301xx s 000 0 eng 10.1007/s004230050256 doi (NATIONALLICENCE)springer-10.1007/s004230050256 DNA polymerases and Ki-67 nuclear antigen are induced in correlation with the resected mass of rat liver up to 90% [Elektronische Daten] [K. Sakaguchi, E. Takeuchi, M. Suzuki, K. Oda, M. Nagino, Y. Nimura, S. Yoshida] Abstract : Background and aims: We studied the regeneration potential by measuring induction of DNA polymerases in the remnant rat liver after a partial hepatectomy (PHx) that is maximal but compatible with survival. Methods: The regenerating rat liver was obtained after the 90% PHx. The induction of activities of DNA polymerase α, δ, and ɛ were measured after partial purification. The Ki-67 nuclear antigen was also detected histochemically. These parameters were compared with those after both 30% and 70% PHx. Results: The 90% hepatectomy resulted in the strong inductions of DNA polymerase α, δ, and ɛ, at 48 h after operation, in association with increases in wet weight and total DNA in the remnant liver. The enzyme induction was much higher after 90% PHx than after 30% and 70% hepatectomy, in correlation with the resection volume. At 48 h after 90% hepatectomy, the Ki-67 positive cells increased up to 47.2% of hepatocytes in the remnant liver. Conclusion: The higher induction of replication enzymes by 90% hepatectomy reflects more cells entering mitogenic cell cycle, which supports the fast regeneration of the remnant liver. The number of proliferating hepatocytes is stringently controlled by an unknown mechanism sensing the mass of resected liver parenchyma. Springer-Verlag Berlin Heidelberg, 2000 Key words Liver regeneration nationallicence DNA polymerase α nationallicence DNA polymerase δ nationallicence DNA polymerase ɛ nationallicence Ki-67 nationallicence Sakaguchi K. First Department of Surgery, Research Institute for Disease Mechanism and Control, Nagoya University School of Medicine, Nagoya 466-8550, Japan, JP aut Takeuchi E. First Department of Surgery, Research Institute for Disease Mechanism and Control, Nagoya University School of Medicine, Nagoya 466-8550, Japan, JP aut Suzuki M. Laboratory of Cancer Cell Biology, Research Institute for Disease Mechanism and Control, Nagoya University School of Medicine, Tsurumai-cho, Showa-ku, Nagoya 466-8550, Japane-mail syoshida@tsuru.med.nagoya-u.ac.jp Tel.: +81-52-7442453 Fax: +81-52-7442457, JP aut Oda K. First Department of Surgery, Research Institute for Disease Mechanism and Control, Nagoya University School of Medicine, Nagoya 466-8550, Japan, JP aut Nagino M. First Department of Surgery, Research Institute for Disease Mechanism and Control, Nagoya University School of Medicine, Nagoya 466-8550, Japan, JP aut Nimura Y. First Department of Surgery, Research Institute for Disease Mechanism and Control, Nagoya University School of Medicine, Nagoya 466-8550, Japan, JP aut Yoshida S. Laboratory of Cancer Cell Biology, Research Institute for Disease Mechanism and Control, Nagoya University School of Medicine, Tsurumai-cho, Showa-ku, Nagoya 466-8550, Japane-mail syoshida@tsuru.med.nagoya-u.ac.jp Tel.: +81-52-7442453 Fax: +81-52-7442457, JP aut https://doi.org/10.1007/s004230050256 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/s004230050256 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Sakaguchi K. First Department of Surgery, Research Institute for Disease Mechanism and Control, Nagoya University School of Medicine, Nagoya 466-8550, Japan, JP aut NATIONALLICENCE 700 1- Takeuchi E. First Department of Surgery, Research Institute for Disease Mechanism and Control, Nagoya University School of Medicine, Nagoya 466-8550, Japan, JP aut NATIONALLICENCE 700 1- Suzuki M. Laboratory of Cancer Cell Biology, Research Institute for Disease Mechanism and Control, Nagoya University School of Medicine, Tsurumai-cho, Showa-ku, Nagoya 466-8550, Japane-mail syoshida@tsuru.med.nagoya-u.ac.jp Tel.: +81-52-7442453 Fax: +81-52-7442457, JP aut NATIONALLICENCE 700 1- Oda K. First Department of Surgery, Research Institute for Disease Mechanism and Control, Nagoya University School of Medicine, Nagoya 466-8550, Japan, JP aut NATIONALLICENCE 700 1- Nagino M. First Department of Surgery, Research Institute for Disease Mechanism and Control, Nagoya University School of Medicine, Nagoya 466-8550, Japan, JP aut NATIONALLICENCE 700 1- Nimura Y. First Department of Surgery, Research Institute for Disease Mechanism and Control, Nagoya University School of Medicine, Nagoya 466-8550, Japan, JP aut NATIONALLICENCE 700 1- Yoshida S. Laboratory of Cancer Cell Biology, Research Institute for Disease Mechanism and Control, Nagoya University School of Medicine, Tsurumai-cho, Showa-ku, Nagoya 466-8550, Japane-mail syoshida@tsuru.med.nagoya-u.ac.jp Tel.: +81-52-7442453 Fax: +81-52-7442457, JP aut Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer