caa a22 4500 475792815 CHVBK 20180406123715.0 cr unu---uuuuu 170329e20001201xx s 000 0 eng 10.1023/A:1011131510491 doi (NATIONALLICENCE)springer-10.1023/A:1011131510491 Detection of Tissue Kallikrein and Kinn B1 and B2 Receptor mRNAs in Human Brain by In Situ RT-PCR [Elektronische Daten] [R. Mahabeer, S. Naidoo, D.M. Raidoo] Tissue kallikrein (TK) and kinin receptors have been immuno-localized in various areas of the human nervous system, suggesting that the kallikrein-kinin system (KKS) may be functionally active in the brain. The aim of this study was to determine the cellular expression of TK and kinin B1 and B2 receptor mRNAs in specific regions of the human brain by in situ reverse transcriptase polymerase chain reaction. Autopsy samples of the brain, spinal cord, kidney and salivary gland were embedded in paraffin. Sections (5μm), adhered onto silane coated glass slides, were treated with Proteinase K and DNase, followed by reverse transcription polymerase chain reaction with specific KKS primers and digoxigenin-dUTP. Detection of the digoxigenin-label demonstrated localization of TK, B1 and B2 mRNAs in the cytoplasm of some neuronal cell bodies in the hypothalamus, thalamus, frontal cortex and spinal cord. TK mRNA was also observed in the ependymal cells lining the cerebral ventricles and epithelial cells of the choroid plexus. In the choroid plexus, only B1 gene expression was observed in some choroidal epithelial cells while no B2 labeling was detected. The identification of mRNAs to TK, B1 and B2 kinin recceptors in human nervous tissue supports previous evidence for the presence of the KKS in the brain and confirms localized protein synthesis. Plenum Publishing Corporation, 2000 Spinal cord nationallicence thalamus nationallicence choroid plexus nationallicence frontal cortex nationallicence digoxigenin-dUTP nationallicence Mahabeer R. Department of Pharmacology, Nelson R. Mandela School of Medicine, University of Natal, South Africa aut Naidoo S. Department of Pharmacology, Nelson R. Mandela School of Medicine, University of Natal, South Africa aut Raidoo D.M. Department of Pharmacology, Nelson R. Mandela School of Medicine, University of Natal, South Africa aut Metabolic Brain Disease Kluwer Academic Publishers-Plenum Publishers 15/4(2000-12-01), 325-335 0885-7490 15:4<325 2000 15 11011 https://doi.org/10.1023/A:1011131510491 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1023/A:1011131510491 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Mahabeer R. Department of Pharmacology, Nelson R. Mandela School of Medicine, University of Natal, South Africa aut NATIONALLICENCE 700 1- Naidoo S. Department of Pharmacology, Nelson R. Mandela School of Medicine, University of Natal, South Africa aut NATIONALLICENCE 700 1- Raidoo D.M. Department of Pharmacology, Nelson R. Mandela School of Medicine, University of Natal, South Africa aut NATIONALLICENCE 773 0- Metabolic Brain Disease Kluwer Academic Publishers-Plenum Publishers 15/4(2000-12-01), 325-335 0885-7490 15:4<325 2000 15 11011 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer