caa a22 4500 475792904 CHVBK 20180406123715.0 cr unu---uuuuu 170329e20001201xx s 000 0 eng 10.1023/A:1011127309582 doi (NATIONALLICENCE)springer-10.1023/A:1011127309582 Development of a Method to Evaluate Glutamate Receptor Function in Rat Barrel Cortex Slices [Elektronische Daten] [Molupe Lehohla, Vivienne Russell, Lauriston Kellaway, Ashnee Govender] The rat is a nocturnal animal and uses its vibrissae extensively to navigate its environment. The vibrissae are linked to a highly organized part of the sensory cortex, called the barrel cortex which contains spiny neurons that receive whisker specific thalamic input and distribute their output mainly within the cortical column. The aim of the present study was to develop a method to evaluate glutamate receptor function in the rat barrel cortex. Long Evans rats (90 - 160g) were killed by cervical dislocation and decapitated. The brain was rapidly removed, cooled in a continuously oxygenated, ice-cold Hepes buffer (pH 7.4) and sliced using a vibratome to produce 0.35mm slices. The barrel cortex was dissected from slices corresponding to 8.6 to 4.8mm anterior to the interaural line and divided into rostral, middle and caudal regions. Depolarization-induced uptake of 45Ca2+ was achieved by incubating test slices in a high K+ (62.5mM) buffer for 2 minutes at 35°C. Potassium-stimulated uptake of 45Ca2+ into the rostral region was significantly lower than into middle and caudal regions of the barrel cortex. Glutamate had no effect. NMDA significantly increased uptake of 45Ca2+ into all regions of the barrel cortex. The technique is useful in determining NMDA receptor function and will be applied to study differences between spontaneously hypertensive rats (SHR) that are used as a model for attention deficit disorder and their normotensive control rats. Plenum Publishing Corporation, 2000 glutamate nationallicence receptor nationallicence NMDA nationallicence barrel cortex nationallicence calcium uptake nationallicence K+ stimulated uptake nationallicence Lehohla Molupe Department of Physiology, Faculty of Health Sciences, University of Cape Town, 7925, Observatory, South Africa aut Russell Vivienne Department of Physiology, Faculty of Health Sciences, University of Cape Town, 7925, Observatory, South Africa aut Kellaway Lauriston Department of Physiology, Faculty of Health Sciences, University of Cape Town, 7925, Observatory, South Africa aut Govender Ashnee Department of Physiology, Faculty of Health Sciences, University of Cape Town, 7925, Observatory, South Africa aut Metabolic Brain Disease Kluwer Academic Publishers-Plenum Publishers 15/4(2000-12-01), 305-314 0885-7490 15:4<305 2000 15 11011 https://doi.org/10.1023/A:1011127309582 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1023/A:1011127309582 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Lehohla Molupe Department of Physiology, Faculty of Health Sciences, University of Cape Town, 7925, Observatory, South Africa aut NATIONALLICENCE 700 1- Russell Vivienne Department of Physiology, Faculty of Health Sciences, University of Cape Town, 7925, Observatory, South Africa aut NATIONALLICENCE 700 1- Kellaway Lauriston Department of Physiology, Faculty of Health Sciences, University of Cape Town, 7925, Observatory, South Africa aut NATIONALLICENCE 700 1- Govender Ashnee Department of Physiology, Faculty of Health Sciences, University of Cape Town, 7925, Observatory, South Africa aut NATIONALLICENCE 773 0- Metabolic Brain Disease Kluwer Academic Publishers-Plenum Publishers 15/4(2000-12-01), 305-314 0885-7490 15:4<305 2000 15 11011 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer