caa a22 4500 475792912 CHVBK 20180406123715.0 cr unu---uuuuu 170329e20001201xx s 000 0 eng 10.1023/A:1011175225512 doi (NATIONALLICENCE)springer-10.1023/A:1011175225512 Increased Glutamate-Stimulated Norepinephrine Release from Prefrontal Cortex Slices of Spontaneously Hypertensive Rats [Elektronische Daten] [V.A. Russell, T.M. Wiggins] Spontaneously hypertensive rats (SHR) have behavioral characteristics (hyperactivity, impulsiveness, poorly sustained attention) similar to the behavioral disturbances of children with attention-deficit hyperactivity disorder (ADHD). We have previously shown that dopaminergic and noradrenergic systems are disturbed in the prefrontal cortex of SHR compared to their normotensive Wistar-Kyoto (WKY) control rats. It was of interest to determine whether the underlying neural circuits that use glutamate as a neurotransmitter function normally in the prefrontal cortex of SHR. An in vitro superfusion technique was used to demonstrate that glutamate caused a concentration-dependent stimulation of [3H]norepinephrine release from rat prefrontal cortex slices. Glutamate (100 μM and 1 mM) caused significantly greater release of norepinephrine from prefrontal cortex slices of SHR than from control slices. The effect of glutamate was not mediated by NMDA receptors, since NMDA (10 and 100 μM) did not exert any effect on norepinephrine release and MK-801 (10 μM) did not antagonize the effect of 100 μM glutamate. These results demonstrate that glutamate stimulates norepinephrine release from rat prefrontal cortex slices and that this increase is enhanced in SHR. The results are consistent with the suggestion that the noradrenergic system is overactive in prefrontal cortex of SHR, the animal model for ADHD. Plenum Publishing Corporation, 2000 noradrenaline nationallicence frontal cortex nationallicence attention nationallicence deficit nationallicence hyperactive nationallicence impulsiveness nationallicence Russell V.A. Department of Physiology, University of Cape Town, Faculty of Health Sciences, Observatory 7925, Cape Town, South Africa aut Wiggins T.M. Department of Physiology, University of Cape Town, Faculty of Health Sciences, Observatory 7925, Cape Town, South Africa aut Metabolic Brain Disease Kluwer Academic Publishers-Plenum Publishers 15/4(2000-12-01), 297-304 0885-7490 15:4<297 2000 15 11011 https://doi.org/10.1023/A:1011175225512 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1023/A:1011175225512 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Russell V.A. Department of Physiology, University of Cape Town, Faculty of Health Sciences, Observatory 7925, Cape Town, South Africa aut NATIONALLICENCE 700 1- Wiggins T.M. Department of Physiology, University of Cape Town, Faculty of Health Sciences, Observatory 7925, Cape Town, South Africa aut NATIONALLICENCE 773 0- Metabolic Brain Disease Kluwer Academic Publishers-Plenum Publishers 15/4(2000-12-01), 297-304 0885-7490 15:4<297 2000 15 11011 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer