caa a22 4500 475792955 CHVBK 20180406123715.0 cr unu---uuuuu 170329e20000601xx s 000 0 eng 10.1007/BF02679976 doi (NATIONALLICENCE)springer-10.1007/BF02679976 A quantitative evaluation of the permeability of the blood brain barrier of portacaval shunted rats [Elektronische Daten] [B. Alexander, X. Li, I. Benjamin, M. Segal, R. Sherwood, J. Preston] The integrity of the blood-brain barrier (BBB) was measured in male Sprague Dawley rats subjected to 16 weeks of portacaval shunting (PCS), the optimal time required for the cerebral changes to develop, by using anin situ brain perfusion technique. The penetration of a vascular space marker14C mannitol, and labelled amino acids3H-phenylalanine or3H-glutamate were measured in brain and cerebrospinal fluid (CSF) using anin situ brain perfusion technique, over 2 or 20 minutes. The patency of the surgical shunt was confirmed by measurement of significantly increased plasma ammonia (131.5±14.8 μ mol.l−1) and AST (159.5±19.9 IU.l−1) concentrations compared to controls 39.9±3.7*, and 82.5±6.6* respectively. Brain and CSF14C-mannitol space (ml. 100g−1), was not increased by PCS where brain space was 1.31±0.27 mL. 100g−1 compared to control 1.19±0.49 mL. 100g, control n=8). The uptake for3H-glutamate, which is required for cerebral ammonia detoxification, was also unchanged in both brain and CSF. However, brain uptake of3H-phenylalanine was significantly reduced from 871 ±80 μL. min−1.g−1 to 356±154* μl.min−1.g−1 (n=4), although there was no change in CSF uptake. These data suggest that there is no generalized breakdown of the blood-brain or blood-CSF barriers during PCS as assessed by mannitol penetration. The reduction in phenylalanine uptake into the brain may help stabilize high cerebral aromatic amino acid levels.*P<0.05, Two-tailed, Student's unpaired t-test. Plenum Publishing Corporation, 2000 Blood-brain barrier nationallicence portal-systemic encephalopathy nationallicence portacaval anastomosis nationallicence amino acids nationallicence Alexander B. Liver Sciences Unit. Academic Department of Surgery, St Thomas' Hospital, Lambeth Palace Road, SE1 7EH, London, UK aut Li X. Liver Sciences Unit. Academic Department of Surgery, St Thomas' Hospital, Lambeth Palace Road, SE1 7EH, London, UK aut Benjamin I. Liver Sciences Unit. Academic Department of Surgery, St Thomas' Hospital, Lambeth Palace Road, SE1 7EH, London, UK aut Segal M. Sherrington School of Physiology, GKT School of Medicine & Dentistry, St Thomas' Hospital, Lambeth Palace Road, SE1 7EH, London, UK aut Sherwood R. Department of Clinical Biochemistry, King's College School of Medicine & Dentistry, Bessemer Road, SE5 9PJ, London aut Preston J. Institute of Gerontology, King's College, London aut Metabolic Brain Disease Springer US 15/2(2000-06-01), 93-103 0885-7490 15:2<93 2000 15 11011 https://doi.org/10.1007/BF02679976 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/BF02679976 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Alexander B. Liver Sciences Unit. Academic Department of Surgery, St Thomas' Hospital, Lambeth Palace Road, SE1 7EH, London, UK aut NATIONALLICENCE 700 1- Li X. Liver Sciences Unit. Academic Department of Surgery, St Thomas' Hospital, Lambeth Palace Road, SE1 7EH, London, UK aut NATIONALLICENCE 700 1- Benjamin I. Liver Sciences Unit. Academic Department of Surgery, St Thomas' Hospital, Lambeth Palace Road, SE1 7EH, London, UK aut NATIONALLICENCE 700 1- Segal M. Sherrington School of Physiology, GKT School of Medicine & Dentistry, St Thomas' Hospital, Lambeth Palace Road, SE1 7EH, London, UK aut NATIONALLICENCE 700 1- Sherwood R. Department of Clinical Biochemistry, King's College School of Medicine & Dentistry, Bessemer Road, SE5 9PJ, London aut NATIONALLICENCE 700 1- Preston J. Institute of Gerontology, King's College, London aut NATIONALLICENCE 773 0- Metabolic Brain Disease Springer US 15/2(2000-06-01), 93-103 0885-7490 15:2<93 2000 15 11011 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer