caa a22 4500 475793447 CHVBK 20180406123716.0 cr unu---uuuuu 170329e20001101xx s 000 0 eng 10.1007/s001220051588 doi (NATIONALLICENCE)springer-10.1007/s001220051588 Two additive QTLs conferring broad-spectrum resistance in potato to Globodera pallida are localized on resistance gene clusters [Elektronische Daten] [J. Rouppe van der Voort, E. van der Vossen, E. Bakker, H. Overmars, P. van Zandvoort, R. Hutten, R. Klein Lankhorst, J. Bakker] Abstract : Broad-spectrum resistance in potato to the potato cyst nematode (PCN) is commonly regarded as a complex inherited trait. Yet, in this paper we show that, by use of a selected set of PCN test populations, broad-spectrum resistance to the species Globodera pallida can be fully ascribed to the action of two loci: Gpa5 and Gpa6. These loci were readily mapped by means of a strategy based on two steps. Firstly, the chromosomal localization of both loci was assessed by use of an online catalogue of AFLP markers covering a substantial part of the potato genome (http://www.spg.wau.nl/pv/aflp/catalog.htm). Subsequently the chromosomal regions of both loci were identified by means of CAPS markers based on RFLP insert sequences. Locus Gpa5 explains at least 61% of the genetic variation. This locus maps to chromosome 5 on a region which has previously been shown to harbor resistance factors to viral (Nb, Rx2), fungal (R1) and nematodal (Gpa, Grp1) pathogens. The Gpa6 locus exhibits a minor effect on the resistance (24%) and acts additively to Gpa5. Interestingly, the Gpa6 locus maps to a region on chromosome 9 where, in the homoeologous tomato genome, the virus resistance gene Sw-5 resides as part of a resistance gene cluster. In potato, resistance to potato virus X has been reported in the vicinity of this region. The map location of Gpa6 indicates the presence of a resistance gene cluster at the end of the long arm of chromosome 9 of potato. Springer-Verlag Berlin Heidelberg, 2000 Key words AFLP nationallicence CAPS nationallicence G. rostochiensis nationallicence Solanum tuberosum nationallicence QTL mapping nationallicence Rouppe van der Voort J. Department of Nematology, The Graduate School of Experimental Plant Sciences, Wageningen Agricultural University, P.O. Box 8123, 6700 ES Wageningen, The Netherlands e-mail: erin.bakker@medew.nema.wau.nl Tel: +31 317 482 197/485 085, Fax: +31 317 484 254, Present address: J. Rouppe van der Voort, KeyGene N.V., P.O. Box 216, 6700 AE, Wageningen, The Netherlands, NL aut van der Vossen E. Centre for Plant Breeding and Reproduction Research, P.O. Box 16, 6700 AA, Wageningen, The Netherlands, NL aut Bakker E. Department of Nematology, The Graduate School of Experimental Plant Sciences, Wageningen Agricultural University, P.O. Box 8123, 6700 ES Wageningen, The Netherlands e-mail: erin.bakker@medew.nema.wau.nl Tel: +31 317 482 197/485 085, Fax: +31 317 484 254, Present address: J. Rouppe van der Voort, KeyGene N.V., P.O. Box 216, 6700 AE, Wageningen, The Netherlands, NL aut Overmars H. Department of Nematology, The Graduate School of Experimental Plant Sciences, Wageningen Agricultural University, P.O. Box 8123, 6700 ES Wageningen, The Netherlands e-mail: erin.bakker@medew.nema.wau.nl Tel: +31 317 482 197/485 085, Fax: +31 317 484 254, Present address: J. Rouppe van der Voort, KeyGene N.V., P.O. Box 216, 6700 AE, Wageningen, The Netherlands, NL aut van Zandvoort P. Department of Nematology, The Graduate School of Experimental Plant Sciences, Wageningen Agricultural University, P.O. Box 8123, 6700 ES Wageningen, The Netherlands e-mail: erin.bakker@medew.nema.wau.nl Tel: +31 317 482 197/485 085, Fax: +31 317 484 254, Present address: J. Rouppe van der Voort, KeyGene N.V., P.O. Box 216, 6700 AE, Wageningen, The Netherlands, NL aut Hutten R. Department of Plant Breeding, The Graduate School of Experimental Plant Sciences, Wageningen Agricultural University, P.O. Box 386, 6700 AJ, Wageningen, The Netherlands, NL aut Klein Lankhorst R. Centre for Plant Breeding and Reproduction Research, P.O. Box 16, 6700 AA, Wageningen, The Netherlands, NL aut Bakker J. Department of Nematology, The Graduate School of Experimental Plant Sciences, Wageningen Agricultural University, P.O. Box 8123, 6700 ES Wageningen, The Netherlands e-mail: erin.bakker@medew.nema.wau.nl Tel: +31 317 482 197/485 085, Fax: +31 317 484 254, Present address: J. Rouppe van der Voort, KeyGene N.V., P.O. Box 216, 6700 AE, Wageningen, The Netherlands, NL aut https://doi.org/10.1007/s001220051588 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/s001220051588 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Rouppe van der Voort J. Department of Nematology, The Graduate School of Experimental Plant Sciences, Wageningen Agricultural University, P.O. Box 8123, 6700 ES Wageningen, The Netherlands e-mail: erin.bakker@medew.nema.wau.nl Tel: +31 317 482 197/485 085, Fax: +31 317 484 254, Present address: J. Rouppe van der Voort, KeyGene N.V., P.O. Box 216, 6700 AE, Wageningen, The Netherlands, NL aut NATIONALLICENCE 700 1- van der Vossen E. Centre for Plant Breeding and Reproduction Research, P.O. Box 16, 6700 AA, Wageningen, The Netherlands, NL aut NATIONALLICENCE 700 1- Bakker E. Department of Nematology, The Graduate School of Experimental Plant Sciences, Wageningen Agricultural University, P.O. Box 8123, 6700 ES Wageningen, The Netherlands e-mail: erin.bakker@medew.nema.wau.nl Tel: +31 317 482 197/485 085, Fax: +31 317 484 254, Present address: J. Rouppe van der Voort, KeyGene N.V., P.O. Box 216, 6700 AE, Wageningen, The Netherlands, NL aut NATIONALLICENCE 700 1- Overmars H. Department of Nematology, The Graduate School of Experimental Plant Sciences, Wageningen Agricultural University, P.O. Box 8123, 6700 ES Wageningen, The Netherlands e-mail: erin.bakker@medew.nema.wau.nl Tel: +31 317 482 197/485 085, Fax: +31 317 484 254, Present address: J. Rouppe van der Voort, KeyGene N.V., P.O. Box 216, 6700 AE, Wageningen, The Netherlands, NL aut NATIONALLICENCE 700 1- van Zandvoort P. Department of Nematology, The Graduate School of Experimental Plant Sciences, Wageningen Agricultural University, P.O. Box 8123, 6700 ES Wageningen, The Netherlands e-mail: erin.bakker@medew.nema.wau.nl Tel: +31 317 482 197/485 085, Fax: +31 317 484 254, Present address: J. Rouppe van der Voort, KeyGene N.V., P.O. Box 216, 6700 AE, Wageningen, The Netherlands, NL aut NATIONALLICENCE 700 1- Hutten R. Department of Plant Breeding, The Graduate School of Experimental Plant Sciences, Wageningen Agricultural University, P.O. Box 386, 6700 AJ, Wageningen, The Netherlands, NL aut NATIONALLICENCE 700 1- Klein Lankhorst R. Centre for Plant Breeding and Reproduction Research, P.O. Box 16, 6700 AA, Wageningen, The Netherlands, NL aut NATIONALLICENCE 700 1- Bakker J. Department of Nematology, The Graduate School of Experimental Plant Sciences, Wageningen Agricultural University, P.O. Box 8123, 6700 ES Wageningen, The Netherlands e-mail: erin.bakker@medew.nema.wau.nl Tel: +31 317 482 197/485 085, Fax: +31 317 484 254, Present address: J. Rouppe van der Voort, KeyGene N.V., P.O. Box 216, 6700 AE, Wageningen, The Netherlands, NL aut Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer