caa a22 4500 475793463 CHVBK 20180406123716.0 cr unu---uuuuu 170329e20001101xx s 000 0 eng 10.1007/s001220051579 doi (NATIONALLICENCE)springer-10.1007/s001220051579 Assessment of genetic diversity in a Morus germplasm collection using fluorescence-based AFLP markers [Elektronische Daten] [A. Sharma, R. Sharma, H. Machii] Abstract : To meet various breeding objectives and to conserve the existing genetic resources of mulberry for future use, the present study was undertaken to investigate the amount of genetic diversity and to establish the relationships between mulberry genotypes using fluorescence-based AFLP markers. Genetic diversity was estimated in 45 mulberry accessions from different eco-geographic regions of Japan and other parts of the world. Five primer combinations amplified an average of 110 AFLP markers per primer combination, ranging in size from 35 to 500 bp. A high degree of polymorphism was revealed by these combinations that ranged from 69.7 to 82.3% across all the genotypes studied. Several rare genotype-specific bands were also identified which could be effectively utilized to distinguish different genotypes. The wide range in genetic similarity coefficients (0.58-0.99) indicated that the mulberry germplasm collection represents a genetically diverse popu-lation. The phenetic dendrogram generated by the UPGMA method grouped 45 accessions into four major clusters, which was in agreement with the results from conventional methods. Clustering of some genotypes into strictly separate groups was not readily apparent and no clear interrelationships could be depicted, in spite of their different geographic origin. In addition, AFLP analysis provided sufficient polymorphism for DNA typing and contributed additional insights into the genetic structure of the mulberry germplasm. These results will help in the formulation of appropriate strategies for conservation and variety improvement in mulberry, for which little or no knowledge of genetic diversity is currently available. Springer-Verlag Berlin Heidelberg, 2000 Key words Mulberry nationallicence Germplasm nationallicence Fluorescence-based AFLP nationallicence Cluster analysis nationallicence Genetic similarity nationallicence Genetic diversity nationallicence Sharma A. National Institute of Sericultural and Entomological Science (NISES), 1-2 Owashi, Tsukuba, Ibaraki-305-8634, Japan e-mail: hmachii@nises.affrc.go.jp Fax: +81-298-38-6028, JP aut Sharma R. National Institute of Sericultural and Entomological Science (NISES), 1-2 Owashi, Tsukuba, Ibaraki-305-8634, Japan e-mail: hmachii@nises.affrc.go.jp Fax: +81-298-38-6028, JP aut Machii H. National Institute of Sericultural and Entomological Science (NISES), 1-2 Owashi, Tsukuba, Ibaraki-305-8634, Japan e-mail: hmachii@nises.affrc.go.jp Fax: +81-298-38-6028, JP aut https://doi.org/10.1007/s001220051579 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/s001220051579 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Sharma A. National Institute of Sericultural and Entomological Science (NISES), 1-2 Owashi, Tsukuba, Ibaraki-305-8634, Japan e-mail: hmachii@nises.affrc.go.jp Fax: +81-298-38-6028, JP aut NATIONALLICENCE 700 1- Sharma R. National Institute of Sericultural and Entomological Science (NISES), 1-2 Owashi, Tsukuba, Ibaraki-305-8634, Japan e-mail: hmachii@nises.affrc.go.jp Fax: +81-298-38-6028, JP aut NATIONALLICENCE 700 1- Machii H. National Institute of Sericultural and Entomological Science (NISES), 1-2 Owashi, Tsukuba, Ibaraki-305-8634, Japan e-mail: hmachii@nises.affrc.go.jp Fax: +81-298-38-6028, JP aut Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer