caa a22 4500 475793471 CHVBK 20180406123716.0 cr unu---uuuuu 170329e20001101xx s 000 0 eng 10.1007/s001220051586 doi (NATIONALLICENCE)springer-10.1007/s001220051586 Detection and cloning of expressed sequences linked to a target gene [Elektronische Daten] [C. E. Vallejos, J. J. Malandro, K. Sheehy, M. J. Zimmermann] Abstract : DNA markers tightly linked to a target gene are essential starting points for positional cloning. We combined ”differential display of mRNA” and ”bulked segregant analysis” in order to detect and clone ten expressed sequences as markers linked to a virus resistance gene in Phaseolus vulgaris. The combination of these two procedures could be used in lieu of positional cloning, provided polymorphisms detectable by differential display exist in the target gene. Isolation of expressed sequences from specific chromosome regions can also be accomplished by combining these procedures. Springer-Verlag Berlin Heidelberg, 2000 Key words Differential display of mRNA nationallicence Bulked segregant analysis nationallicence Bean common mosaic virus nationallicence Linkage analysis nationallicence Vallejos C. E. Department of Horticultural Sciences, and Plant Molecular and Cellular Biology Graduate Program, University of Florida, 1143 Fifield Hall, Gainesville, FL 32611, USA e-mail: Vallejos@ufl.edu, Fax: +1 353 392-6479, US aut Malandro J. J. Department of Horticultural Sciences, and Plant Molecular and Cellular Biology Graduate Program, University of Florida, 1143 Fifield Hall, Gainesville, FL 32611, USA e-mail: Vallejos@ufl.edu, Fax: +1 353 392-6479, US aut Sheehy K. Department of Horticultural Sciences, and Plant Molecular and Cellular Biology Graduate Program, University of Florida, 1143 Fifield Hall, Gainesville, FL 32611, USA e-mail: Vallejos@ufl.edu, Fax: +1 353 392-6479, US aut Zimmermann M. J. Centro Nacional de Pesquisa de Arroz e Feijao, Empresa Brasileira de Pesquisa Agropecuaria, Goiânia, GO, Brazil, Present address: FAO-SDRR, Office: C-692, Via delle Terme di Caracalla, 00100-Rome, Italy, BR aut https://doi.org/10.1007/s001220051586 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/s001220051586 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Vallejos C. E. Department of Horticultural Sciences, and Plant Molecular and Cellular Biology Graduate Program, University of Florida, 1143 Fifield Hall, Gainesville, FL 32611, USA e-mail: Vallejos@ufl.edu, Fax: +1 353 392-6479, US aut NATIONALLICENCE 700 1- Malandro J. J. Department of Horticultural Sciences, and Plant Molecular and Cellular Biology Graduate Program, University of Florida, 1143 Fifield Hall, Gainesville, FL 32611, USA e-mail: Vallejos@ufl.edu, Fax: +1 353 392-6479, US aut NATIONALLICENCE 700 1- Sheehy K. Department of Horticultural Sciences, and Plant Molecular and Cellular Biology Graduate Program, University of Florida, 1143 Fifield Hall, Gainesville, FL 32611, USA e-mail: Vallejos@ufl.edu, Fax: +1 353 392-6479, US aut NATIONALLICENCE 700 1- Zimmermann M. J. Centro Nacional de Pesquisa de Arroz e Feijao, Empresa Brasileira de Pesquisa Agropecuaria, Goiânia, GO, Brazil, Present address: FAO-SDRR, Office: C-692, Via delle Terme di Caracalla, 00100-Rome, Italy, BR aut Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer