caa a22 4500 475793498 CHVBK 20180406123717.0 cr unu---uuuuu 170329e20001101xx s 000 0 eng 10.1007/s001220051576 doi (NATIONALLICENCE)springer-10.1007/s001220051576 Characterization and detection of epistatic interactions of 3 QTLs, Hd1, Hd2, and Hd3, controlling heading date in rice using nearly isogenic lines [Elektronische Daten] [H. X. Lin, T. Yamamoto, T. Sasaki, M. Yano] Abstract : To characterize quantitative trait loci (QTLs), we used marker-assisted selection (MAS) to develop three nearly isogenic lines (NILs) differing only for the presence of a single, specific QTL (QTL-NILs) -Hd1, Hd2, and Hd3 - for heading date in rice. The three lines contained the chromosomal region of the target QTL from donor variety Kasalath(indica) in the genetic background of var. Nipponbare (japonica). To analyze epistatic interactions in pairs of these QTLs, we also used MAS to develop four combined QTL-NILs with 2 of the 3 QTLs or with all 3. Different daylength treatment testing of the QTL-NILs revealed that the three QTLs control photoperiod sensitivity. Genetic analysis of F2 populations derived from crosses between the three QTL-NILs with a single QTL using molecular markers revealed the existence of epistatic interactions between Hd1 and Hd2, and Hd2 and Hd3. These interactions were also confirmed by the analysis of combined QTL-NILs under different daylength conditions. The existence of an epistatic interaction between Hd1 and Hd3 was also clarified. Based on these results, we suggest that the Kasalath allele of Hd3 does not affect photoperiod sensitivity by itself but that it is involved in enhancement of the expression of the Nipponbare alleles of Hd1 and Hd2. Springer-Verlag Berlin Heidelberg, 2000 Key words Oryza sativa L nationallicence Quantitative trait loci nationallicence Photoperiod sensitivity nationallicence Digenic interaction nationallicence Nearly isogenic lines nationallicence Lin H. X. Bio-oriented Technology Research Advancement Institution, Omiya, Saitama 331-8537, Japan, JP aut Yamamoto T. Institute of the Society for Techno-innovation of Agriculture, Forestry and Fisheries, Tsukuba, Ibaraki 305-0854, Japan, Present address: Orynova K. K., Toyoda, Iwata, Shizuoka, 438-0802, Japan, JP aut Sasaki T. Department of Molecular Genetics, National Institute of Agrobiological Resources, Tsukuba, Ibaraki 305-8602, Japan e-mail: myano@abr.affrc.go.jp Fax: +81 298-38-7468, JP aut Yano M. Department of Molecular Genetics, National Institute of Agrobiological Resources, Tsukuba, Ibaraki 305-8602, Japan e-mail: myano@abr.affrc.go.jp Fax: +81 298-38-7468, JP aut https://doi.org/10.1007/s001220051576 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/s001220051576 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Lin H. X. Bio-oriented Technology Research Advancement Institution, Omiya, Saitama 331-8537, Japan, JP aut NATIONALLICENCE 700 1- Yamamoto T. Institute of the Society for Techno-innovation of Agriculture, Forestry and Fisheries, Tsukuba, Ibaraki 305-0854, Japan, Present address: Orynova K. K., Toyoda, Iwata, Shizuoka, 438-0802, Japan, JP aut NATIONALLICENCE 700 1- Sasaki T. Department of Molecular Genetics, National Institute of Agrobiological Resources, Tsukuba, Ibaraki 305-8602, Japan e-mail: myano@abr.affrc.go.jp Fax: +81 298-38-7468, JP aut NATIONALLICENCE 700 1- Yano M. Department of Molecular Genetics, National Institute of Agrobiological Resources, Tsukuba, Ibaraki 305-8602, Japan e-mail: myano@abr.affrc.go.jp Fax: +81 298-38-7468, JP aut Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer