caa a22 4500 475793501 CHVBK 20180406123717.0 cr unu---uuuuu 170329e20001101xx s 000 0 eng 10.1007/s001220051575 doi (NATIONALLICENCE)springer-10.1007/s001220051575 Shorter T-DNA or additional virulence genes improve Agrobactrium-mediated transformation [Elektronische Daten] [S. H. Park, B.-M. Lee, M. G. Salas, M. Srivatanakul, R. H. Smith] Abstract : The effect of additional virulence (vir) genes and size of plasmid T-DNA in Agrobacterium tumefa- ciens was investigated for their impact on transformation efficiency. Transformation efficiency in tobacco, cotton, and rice was increased when the T-DNA was 4.3 kb compared to 8.4 kb in size. However, when additional virG, virGN54D,virE, or virE/virG plasmids were included with the 8.4-kb T-DNA, transformation frequencies in all cases were increased over that of the shorter T-DNA without additional vir plasmids. The use of virE, virG or virGN54D copies enhanced transformation efficiency; however, the most significant increase of transformation efficiency in all three plant species was observed when the virE/virG plasmid was used for infection. The virE/virG plasmid dramatically enhanced the efficiency of Agrobacterium-mediated gene transfer; moreover, this plasmid appears to have broad efficiency since it was consistently effective on two different dicotyledon species as well as a monocotyledon species. Springer-Verlag Berlin Heidelberg, 2000 Key words Agrobacterium nationallicence Rice nationallicence Cotton nationallicence Tobacco nationallicence Virulence genes nationallicence Park S. H. Department of Soil and Crop Sciences, Texas A&M University, College Station, TX 77843, USA Fax: +1 409-862-4301 e-mail: rsmith@tamu.edu, US aut Lee B.-M Department of Soil and Crop Sciences, Texas A&M University, College Station, TX 77843, USA Fax: +1 409-862-4301 e-mail: rsmith@tamu.edu, US aut Salas M. G. Department of Soil and Crop Sciences, Texas A&M University, College Station, TX 77843, USA Fax: +1 409-862-4301 e-mail: rsmith@tamu.edu, US aut Srivatanakul M. Department of Soil and Crop Sciences, Texas A&M University, College Station, TX 77843, USA Fax: +1 409-862-4301 e-mail: rsmith@tamu.edu, US aut Smith R. H. Department of Soil and Crop Sciences, Texas A&M University, College Station, TX 77843, USA Fax: +1 409-862-4301 e-mail: rsmith@tamu.edu, US aut https://doi.org/10.1007/s001220051575 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/s001220051575 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Park S. H. Department of Soil and Crop Sciences, Texas A&M University, College Station, TX 77843, USA Fax: +1 409-862-4301 e-mail: rsmith@tamu.edu, US aut NATIONALLICENCE 700 1- Lee B.-M Department of Soil and Crop Sciences, Texas A&M University, College Station, TX 77843, USA Fax: +1 409-862-4301 e-mail: rsmith@tamu.edu, US aut NATIONALLICENCE 700 1- Salas M. G. Department of Soil and Crop Sciences, Texas A&M University, College Station, TX 77843, USA Fax: +1 409-862-4301 e-mail: rsmith@tamu.edu, US aut NATIONALLICENCE 700 1- Srivatanakul M. Department of Soil and Crop Sciences, Texas A&M University, College Station, TX 77843, USA Fax: +1 409-862-4301 e-mail: rsmith@tamu.edu, US aut NATIONALLICENCE 700 1- Smith R. H. Department of Soil and Crop Sciences, Texas A&M University, College Station, TX 77843, USA Fax: +1 409-862-4301 e-mail: rsmith@tamu.edu, US aut Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer