caa a22 4500 47579351X CHVBK 20180406123717.0 cr unu---uuuuu 170329e20001101xx s 000 0 eng 10.1007/s001220051585 doi (NATIONALLICENCE)springer-10.1007/s001220051585 RFLP mapping of manganese efficiency in barley [Elektronische Daten] [M. A. Pallotta, R. D. Graham, P. Langridge, D. H. B. Sparrow, S. J. Barker] Abstract : In many cropping regions of the world, yield is limited by the availability of micronutrients, and micronutrient-efficient cultivars provide a yield advantage. Traditional methods of testing cultivars for micronutrient efficiency are time-consuming and laborious. Molecular markers linked to loci controlling micronutrient efficiency will allow more rapid and efficient selection and introgression of these traits than is currently possible. Using a pot-based bioassay and bulked segregant analysis of an F2 population, we have identified several RFLPs (grouped distally on chromosome 4HS) linked to a locus for manganese efficiency in barley. This manganese efficiency locus has been designated Mel1. Pot bioassay analysis of intercrosses suggests that three useful sources of manganese efficiency are likely to be allelic at the Mel1 locus. Field evaluation of marker selected F4 progeny supports the major role of Mel1 in the genetic control of manganese efficiency. Adoption of marker assisted selection for this trait in the Southern Australian barley breeding program has occurred. This has been facilitated by the demonstration that the Mel1 allele of Amagi Nijo can be distinguished from 95 other locally useful varieties and breeder's lines on the basis of RFLPs identified by just two molecular markers. Springer-Verlag Berlin Heidelberg, 2000 Key words Barley nationallicence Manganese efficiency nationallicence RFLP mapping nationallicence Marker-assisted Selection nationallicence Plant nutrition nationallicence 4HS nationallicence Pallotta M. A. Department of Plant Science, The University of Adelaide, Waite Campus, Urrbrae, South Australia, Australia 5064 Fax: +61 8 8303 7109 e-mail: margaret.pallotta@adelaide.edu.au, AU aut Graham R. D. Department of Plant Science, The University of Adelaide, Waite Campus, Urrbrae, South Australia, Australia 5064 Fax: +61 8 8303 7109 e-mail: margaret.pallotta@adelaide.edu.au, AU aut Langridge P. Department of Plant Science, The University of Adelaide, Waite Campus, Urrbrae, South Australia, Australia 5064 Fax: +61 8 8303 7109 e-mail: margaret.pallotta@adelaide.edu.au, AU aut Sparrow D. H. B. Department of Plant Science, The University of Adelaide, Waite Campus, Urrbrae, South Australia, Australia 5064 Fax: +61 8 8303 7109 e-mail: margaret.pallotta@adelaide.edu.au, AU aut Barker S. J. Faculty of Agriculture, The University of Western Australia, Nedlands, Western Australia, Australia 6907, AU aut https://doi.org/10.1007/s001220051585 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/s001220051585 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Pallotta M. A. Department of Plant Science, The University of Adelaide, Waite Campus, Urrbrae, South Australia, Australia 5064 Fax: +61 8 8303 7109 e-mail: margaret.pallotta@adelaide.edu.au, AU aut NATIONALLICENCE 700 1- Graham R. D. Department of Plant Science, The University of Adelaide, Waite Campus, Urrbrae, South Australia, Australia 5064 Fax: +61 8 8303 7109 e-mail: margaret.pallotta@adelaide.edu.au, AU aut NATIONALLICENCE 700 1- Langridge P. Department of Plant Science, The University of Adelaide, Waite Campus, Urrbrae, South Australia, Australia 5064 Fax: +61 8 8303 7109 e-mail: margaret.pallotta@adelaide.edu.au, AU aut NATIONALLICENCE 700 1- Sparrow D. H. B. Department of Plant Science, The University of Adelaide, Waite Campus, Urrbrae, South Australia, Australia 5064 Fax: +61 8 8303 7109 e-mail: margaret.pallotta@adelaide.edu.au, AU aut NATIONALLICENCE 700 1- Barker S. J. Faculty of Agriculture, The University of Western Australia, Nedlands, Western Australia, Australia 6907, AU aut Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer