caa a22 4500 475793536 CHVBK 20180406123717.0 cr unu---uuuuu 170329e20001101xx s 000 0 eng 10.1007/s001220051589 doi (NATIONALLICENCE)springer-10.1007/s001220051589 Isolation and characterization of the Δ12-fatty acid desaturase in peanut (Arachis hypogaea L.) and search for polymorphisms for the high oleate trait in Spanish market-type lines [Elektronische Daten] [Y. López, H. L. Nadaf, O. D. Smith, J. P. Connell, A. S. Reddy, A. K. Fritz] Abstract : An understanding of the molecular mechanisms that are responsible for increased oleic acid accumulation would open avenues to alter peanut fatty acid composition and allow detection of polymorphic regions which can be used for marker assisted selection (MAS). Δ12-Fatty acid desaturase (FAD) was isolated and characterized from genotypes having a low or high oleic to linoleic acid O/L ratio - genotypes, Tamspan 90 (T-90) and F435-2-2 (F435), respectively. Southern blots showed three to four copies per haploid genome, and no major differences in organization between the two parental lines. Approximately 3525 bp was isolated from both genotypes, including a genomic walk toward the promoter region. The Δ12-Fad contains a putative intron, the coding region at the 3′ end, and an open reading frame (ORF) of 1140 bp encoding 379 amino acids. Comparisons of the coding sequences from the high and low oleic acid genotypes revealed several single nucleotide polymorphisms (SNPs). Two polymorphisms appear to be associated with the high O/L trait. The first is an ”A” insertion 442 bp after the start codon. The ”A” insertion shifts the amino acid reading frame, probably resulting in a truncated, inactive protein and the loss of one of three histidine boxes believed to be involved in metal ion complexation required for the reduction of oxygen. Another polymorphism at 448 bp from the start codon results in an amino acid change. The region containing the polymorphisms was amplified from leaf tissue of several independently derived backcross lines (IDBLs). Most high O/L lines had either the ”A” insertion or the amino acid substitution. Springer-Verlag Berlin Heidelberg, 2000 Key words Arachis hypogaea L nationallicence Δ12-Fatty acid desaturase nationallicence Genomic sequences nationallicence Oleic peanut nationallicence Single nucleotide polymorphisms (SNPs) nationallicence López Y. Department of Soil and Crop Sciences, Texas A&M University, College Station, TX 77843-2474, USA e-mail: y-lopez@Tamu.edu), US aut Nadaf H. L. University of Agricultural Sciences, Dharwad-580005, Karnatuka, India, IN aut Smith O. D. Department of Soil and Crop Sciences, Texas A&M University, College Station, TX 77843-2474, USA e-mail: y-lopez@Tamu.edu), US aut Connell J. P. Department of Soil and Crop Sciences, Texas A&M University, College Station, TX 77843-2474, USA e-mail: y-lopez@Tamu.edu), US aut Reddy A. S. Department of Soil and Crop Sciences, Texas A&M University, College Station, TX 77843-2474, USA e-mail: y-lopez@Tamu.edu), US aut Fritz A. K. Department of Soil and Crop Sciences, Texas A&M University, College Station, TX 77843-2474, USA e-mail: y-lopez@Tamu.edu), US aut https://doi.org/10.1007/s001220051589 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/s001220051589 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- López Y. Department of Soil and Crop Sciences, Texas A&M University, College Station, TX 77843-2474, USA e-mail: y-lopez@Tamu.edu), US aut NATIONALLICENCE 700 1- Nadaf H. L. University of Agricultural Sciences, Dharwad-580005, Karnatuka, India, IN aut NATIONALLICENCE 700 1- Smith O. D. Department of Soil and Crop Sciences, Texas A&M University, College Station, TX 77843-2474, USA e-mail: y-lopez@Tamu.edu), US aut NATIONALLICENCE 700 1- Connell J. P. Department of Soil and Crop Sciences, Texas A&M University, College Station, TX 77843-2474, USA e-mail: y-lopez@Tamu.edu), US aut NATIONALLICENCE 700 1- Reddy A. S. Department of Soil and Crop Sciences, Texas A&M University, College Station, TX 77843-2474, USA e-mail: y-lopez@Tamu.edu), US aut NATIONALLICENCE 700 1- Fritz A. K. Department of Soil and Crop Sciences, Texas A&M University, College Station, TX 77843-2474, USA e-mail: y-lopez@Tamu.edu), US aut Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer