caa a22 4500 475793544 CHVBK 20180406123717.0 cr unu---uuuuu 170329e20001101xx s 000 0 eng 10.1007/s001220051592 doi (NATIONALLICENCE)springer-10.1007/s001220051592 A linkage map of the chickpea (Cicer arietinum L.) genome based on recombinant inbred lines from a C. arietinum×C. reticulatum cross: localization of resistance genes for fusarium wilt races 4 and 5 [Elektronische Daten] [P. Winter, A.-M. Benko-Iseppon, B. Hüttel, M. Ratnaparkhe, A. Tullu, G. Sonnante, T. Pfaff, M. Tekeoglu, D. Santra, V. J. Sant, P. N. Rajesh, G. Kahl, F. J. Muehlbauer] Abstract : An integrated molecular marker map of the chickpea genome was established using 130 recombinant inbred lines from a wide cross between a cultivar resistant to fusarium wilt caused by Fusarium oxysporum Schlecht. emend. Snyd. &. Hans f. sp. ciceri (Padwick) Snyd & Hans, and an accession of Cicer reticulatum (PI 489777), the wild progenitor of chickpea. A total of 354 markers were mapped on the RILs including 118 STMSs, 96 DAFs, 70 AFLPs, 37 ISSRs, 17 RAPDs, eight isozymes, three cDNAs, two SCARs and three loci that confer resistance against different races of fusarium wilt. At a LOD-score of 4.0, 303 markers cover 2077.9 cM in eight large and eight small linkage groups at an average distance of 6.8 cM between markers. Fifty one markers (14.4%) were unlinked. A clustering of markers in central regions of linkage groups was observed. Markers of the same class, except for ISSR and RAPD markers, tended to generate subclusters. Also, genes for resistance to races 4 and 5 of fusarium wilt map to the same linkage group that includes an STMS and a SCAR marker previously shown to be linked to fusarium wilt race 1, indicating a clustering of several fusarium-wilt resistance genes around this locus. Significant deviation from the expected 1 : 1 segregation ratio was observed for 136 markers (38.4%, P<0.05). Segregation was biased towards the wild progenitor in 68% of the cases. Segregation distortion was similar for all marker types except for ISSRs that showed only 28.5% aberrant segregation. The map is the most extended genetic map of chickpea currently available. It may serve as a basis for marker-assisted selection and map-based cloning of fusarium wilt resistance genes and other agronomically important genes in future. Springer-Verlag Berlin Heidelberg, 2000 Key words Chickpea nationallicence Genetic map nationallicence Molecular markers nationallicence Fusarium wilt nationallicence Disease resistance genes nationallicence Winter P. Plant Molecular Biology, Biocentre, University of Frankfurt, Marie-Curie-Strasse 9, D-60439 Frankfurt, Germany e-mail: p.winter@em.uni-frankfurt.de, DE aut Benko-Iseppon A.-M Universidad Federal de Pernambuco, UFPE, CCB, Genética, Av. Prof. Moraes Rego, s/no. 50732-970, Recife - PE, Brazil, BR aut Hüttel B. Plant Molecular Biology, Biocentre, University of Frankfurt, Marie-Curie-Strasse 9, D-60439 Frankfurt, Germany e-mail: p.winter@em.uni-frankfurt.de, DE aut Ratnaparkhe M. U.S. Department of Agriculture and the Department of Crop and Soil Sciences, Washington State University, Pullman, WA 99164-6434, USA, US aut Tullu A. U.S. Department of Agriculture and the Department of Crop and Soil Sciences, Washington State University, Pullman, WA 99164-6434, USA, US aut Sonnante G. CNR Istituto del Germoplasma, Via Amendola 165/A, 70126 BARI, Italy, IT aut Pfaff T. Plant Molecular Biology, Biocentre, University of Frankfurt, Marie-Curie-Strasse 9, D-60439 Frankfurt, Germany e-mail: p.winter@em.uni-frankfurt.de, DE aut Tekeoglu M. U.S. Department of Agriculture and the Department of Crop and Soil Sciences, Washington State University, Pullman, WA 99164-6434, USA, US aut Santra D. U.S. Department of Agriculture and the Department of Crop and Soil Sciences, Washington State University, Pullman, WA 99164-6434, USA, US aut Sant V. J. Plant Molecular Biology Unit, National Chemical Laboratory, Homi Bhabha Road, Pashan, Pune 411008, India, IN aut Rajesh P. N. Plant Molecular Biology Unit, National Chemical Laboratory, Homi Bhabha Road, Pashan, Pune 411008, India, IN aut Kahl G. Plant Molecular Biology, Biocentre, University of Frankfurt, Marie-Curie-Strasse 9, D-60439 Frankfurt, Germany e-mail: p.winter@em.uni-frankfurt.de, DE aut Muehlbauer F. J. U.S. Department of Agriculture and the Department of Crop and Soil Sciences, Washington State University, Pullman, WA 99164-6434, USA, US aut https://doi.org/10.1007/s001220051592 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/s001220051592 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Winter P. Plant Molecular Biology, Biocentre, University of Frankfurt, Marie-Curie-Strasse 9, D-60439 Frankfurt, Germany e-mail: p.winter@em.uni-frankfurt.de, DE aut NATIONALLICENCE 700 1- Benko-Iseppon A.-M Universidad Federal de Pernambuco, UFPE, CCB, Genética, Av. Prof. Moraes Rego, s/no. 50732-970, Recife - PE, Brazil, BR aut NATIONALLICENCE 700 1- Hüttel B. Plant Molecular Biology, Biocentre, University of Frankfurt, Marie-Curie-Strasse 9, D-60439 Frankfurt, Germany e-mail: p.winter@em.uni-frankfurt.de, DE aut NATIONALLICENCE 700 1- Ratnaparkhe M. U.S. Department of Agriculture and the Department of Crop and Soil Sciences, Washington State University, Pullman, WA 99164-6434, USA, US aut NATIONALLICENCE 700 1- Tullu A. U.S. Department of Agriculture and the Department of Crop and Soil Sciences, Washington State University, Pullman, WA 99164-6434, USA, US aut NATIONALLICENCE 700 1- Sonnante G. CNR Istituto del Germoplasma, Via Amendola 165/A, 70126 BARI, Italy, IT aut NATIONALLICENCE 700 1- Pfaff T. Plant Molecular Biology, Biocentre, University of Frankfurt, Marie-Curie-Strasse 9, D-60439 Frankfurt, Germany e-mail: p.winter@em.uni-frankfurt.de, DE aut NATIONALLICENCE 700 1- Tekeoglu M. U.S. Department of Agriculture and the Department of Crop and Soil Sciences, Washington State University, Pullman, WA 99164-6434, USA, US aut NATIONALLICENCE 700 1- Santra D. U.S. Department of Agriculture and the Department of Crop and Soil Sciences, Washington State University, Pullman, WA 99164-6434, USA, US aut NATIONALLICENCE 700 1- Sant V. J. Plant Molecular Biology Unit, National Chemical Laboratory, Homi Bhabha Road, Pashan, Pune 411008, India, IN aut NATIONALLICENCE 700 1- Rajesh P. N. Plant Molecular Biology Unit, National Chemical Laboratory, Homi Bhabha Road, Pashan, Pune 411008, India, IN aut NATIONALLICENCE 700 1- Kahl G. Plant Molecular Biology, Biocentre, University of Frankfurt, Marie-Curie-Strasse 9, D-60439 Frankfurt, Germany e-mail: p.winter@em.uni-frankfurt.de, DE aut NATIONALLICENCE 700 1- Muehlbauer F. J. U.S. Department of Agriculture and the Department of Crop and Soil Sciences, Washington State University, Pullman, WA 99164-6434, USA, US aut Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer