caa a22 4500 475793579 CHVBK 20180406123717.0 cr unu---uuuuu 170329e20001101xx s 000 0 eng 10.1007/s001220051581 doi (NATIONALLICENCE)springer-10.1007/s001220051581 Lettuce, a shallow-rooted crop, and Lactuca serriola, its wild progenitor, differ at QTL determining root architecture and deep soil water exploitation [Elektronische Daten] [W. C. Johnson, L. E. Jackson, O. Ochoa, R. van Wijk, J. Peleman, D. A. St. Clair, R. W. Michelmore] Abstract : Wild plant species are often adapted to more stressful environments than their cultivated relatives. Roots are critical in exploiting soil resources that enable plants to withstand environmental stresses, but they are difficult to study. Cultivated lettuce (Lactuca sativa L.) and wild L. serriola L. differ greatly in both shoot and root characteristics. Approximately 100 F2:3 families derived from an interspecific cross were evaluated in greenhouse and field experiments. In the greenhouse, root traits (taproot length, number of laterals emerging from the taproot, and biomass) and shoot biomass were measured 4 weeks after planting. In the field, plants were grown for 9 weeks (close to harvest maturity of the cultivated parent); mild drought stress was induced by withholding water for 1 week, and gravimetric moisture of soil was then determined for five depth increments between 0-100 cm. The families were genotyped using codominantly scored AFLP markers distributed throughout the genome. Composite interval mapping was used to analyze marker-trait associations. Quantitative trait loci were identified for differences between wild and cultivated lettuce for root architectural traits and water acquisition. Thirteen QTL were detected that each accounted for 28-83% of the phenotypic variation. The loci for taproot length (i.e., cm taproot length g-1 plant biomass) and the ability to extract water from deep in the soil profile co-localized in the genome. These coincident loci were identified in separate experiments. The wild L. serriola is therefore a potential source of agriculturally important alleles to optimize resource acquisition by cultivated lettuce, thereby minimizing water and fertilizer inputs and ultimately enhancing water quality. Springer-Verlag Berlin Heidelberg, 2000 Key words Root architecture nationallicence Soil water nationallicence Quantitative trait loci nationallicence Crop domestication nationallicence AFLP nationallicence Johnson W. C. Department of Vegetable Crops, University of California, One Shields Ave., Davis, CA 95616-8746, USA Fax: +1 530-752-9659 e-mail: rwmichelmore@ucdavis.edu, Present address: W.C. Johnson, USDA-ARS/Department of Horticultural Sciences, Cornell University, Geneva, NY 14456, USA, US aut Jackson L. E. Department of Vegetable Crops, University of California, One Shields Ave., Davis, CA 95616-8746, USA Fax: +1 530-752-9659 e-mail: rwmichelmore@ucdavis.edu, Present address: W.C. Johnson, USDA-ARS/Department of Horticultural Sciences, Cornell University, Geneva, NY 14456, USA, US aut Ochoa O. Department of Vegetable Crops, University of California, One Shields Ave., Davis, CA 95616-8746, USA Fax: +1 530-752-9659 e-mail: rwmichelmore@ucdavis.edu, Present address: W.C. Johnson, USDA-ARS/Department of Horticultural Sciences, Cornell University, Geneva, NY 14456, USA, US aut van Wijk R. Keygene N.V., 6700 AG Wageningen, The Netherlands, NL aut Peleman J. Keygene N.V., 6700 AG Wageningen, The Netherlands, NL aut St. Clair D. A. Department of Vegetable Crops, University of California, One Shields Ave., Davis, CA 95616-8746, USA Fax: +1 530-752-9659 e-mail: rwmichelmore@ucdavis.edu, Present address: W.C. Johnson, USDA-ARS/Department of Horticultural Sciences, Cornell University, Geneva, NY 14456, USA, US aut Michelmore R. W. Department of Vegetable Crops, University of California, One Shields Ave., Davis, CA 95616-8746, USA Fax: +1 530-752-9659 e-mail: rwmichelmore@ucdavis.edu, Present address: W.C. Johnson, USDA-ARS/Department of Horticultural Sciences, Cornell University, Geneva, NY 14456, USA, US aut https://doi.org/10.1007/s001220051581 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/s001220051581 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Johnson W. C. Department of Vegetable Crops, University of California, One Shields Ave., Davis, CA 95616-8746, USA Fax: +1 530-752-9659 e-mail: rwmichelmore@ucdavis.edu, Present address: W.C. Johnson, USDA-ARS/Department of Horticultural Sciences, Cornell University, Geneva, NY 14456, USA, US aut NATIONALLICENCE 700 1- Jackson L. E. Department of Vegetable Crops, University of California, One Shields Ave., Davis, CA 95616-8746, USA Fax: +1 530-752-9659 e-mail: rwmichelmore@ucdavis.edu, Present address: W.C. Johnson, USDA-ARS/Department of Horticultural Sciences, Cornell University, Geneva, NY 14456, USA, US aut NATIONALLICENCE 700 1- Ochoa O. Department of Vegetable Crops, University of California, One Shields Ave., Davis, CA 95616-8746, USA Fax: +1 530-752-9659 e-mail: rwmichelmore@ucdavis.edu, Present address: W.C. Johnson, USDA-ARS/Department of Horticultural Sciences, Cornell University, Geneva, NY 14456, USA, US aut NATIONALLICENCE 700 1- van Wijk R. Keygene N.V., 6700 AG Wageningen, The Netherlands, NL aut NATIONALLICENCE 700 1- Peleman J. Keygene N.V., 6700 AG Wageningen, The Netherlands, NL aut NATIONALLICENCE 700 1- St. Clair D. A. Department of Vegetable Crops, University of California, One Shields Ave., Davis, CA 95616-8746, USA Fax: +1 530-752-9659 e-mail: rwmichelmore@ucdavis.edu, Present address: W.C. Johnson, USDA-ARS/Department of Horticultural Sciences, Cornell University, Geneva, NY 14456, USA, US aut NATIONALLICENCE 700 1- Michelmore R. W. Department of Vegetable Crops, University of California, One Shields Ave., Davis, CA 95616-8746, USA Fax: +1 530-752-9659 e-mail: rwmichelmore@ucdavis.edu, Present address: W.C. Johnson, USDA-ARS/Department of Horticultural Sciences, Cornell University, Geneva, NY 14456, USA, US aut Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer