caa a22 4500 475793595 CHVBK 20180406123717.0 cr unu---uuuuu 170329e20001101xx s 000 0 eng 10.1007/s001220051574 doi (NATIONALLICENCE)springer-10.1007/s001220051574 Development and characterisation of Brassica napus-Sinapis arvensis addition lines exhibiting resistance to Leptosphaeria maculans [Elektronische Daten] [R. J. Snowdon, H. Winter, A. Diestel, M. D. Sacristán] Abstract : Blackleg caused by Leptosphaeria maculans is one of the most important diseases affecting oilseed rape worldwide. Sinapis arvensis is valuable for the transfer of blackleg resistance to oilseed rape (Brassica napus) because this species contains high resistance against various aggressive isolates of the blackleg fungus. These include at least one Australian isolate which has been found to overcome resistance originating from species with the Brassica B genome, until now the major source for interspecific transfer of blackleg resistance. Backcross offspring from intergeneric crosses between Brassica napus and S. arvensis were subjected to phytopathological studies and molecular cytogenetic analysis with genomic in situ hybridisation (GISH). The BC3S progenies included fertile plants exhibiting high seedling (cotyledon) and adult plant resistance associated with the presence of an acrocentric addition chromosome from S. arvensis. In addition, some individuals with adult plant resistance but cotyledon susceptibility were observed to have a normal B. napus karyotype with no visible GISH signals, indicating possible resistant introgression lines. Phytopathological analysis of selfing progenies from 3 different highly resistant BC3 plants showed that seedling and adult plant resistance are probably conferred by different loci. Springer-Verlag Berlin Heidelberg, 2000 Key words Brassica napus nationallicence Sinapis arvensis nationallicence Leptosphaeria maculans nationallicence Intergeneric hybrids nationallicence Blackleg resistance nationallicence Genomic in situ hybridisation nationallicence Snowdon R. J. Institut für Pflanzenbau und Pflanzenzüchtung II, Justus-Liebig-Universität Giessen, IFZ, Heinrich-Buff-Ring 26, 35392 Giessen, Germany, DE aut Winter H. Institut für Biologie - Angewandte Genetik, Freie Universität Berlin, Albrecht-Thaer-Weg 6, 14195 Berlin, Germany Fax: +49 (030) 838 54345 e-mail: sacris@zedat.fu-berlin.de, DE aut Diestel A. Institut für Biologie - Angewandte Genetik, Freie Universität Berlin, Albrecht-Thaer-Weg 6, 14195 Berlin, Germany Fax: +49 (030) 838 54345 e-mail: sacris@zedat.fu-berlin.de, DE aut Sacristán M. D. Institut für Biologie - Angewandte Genetik, Freie Universität Berlin, Albrecht-Thaer-Weg 6, 14195 Berlin, Germany Fax: +49 (030) 838 54345 e-mail: sacris@zedat.fu-berlin.de, DE aut https://doi.org/10.1007/s001220051574 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/s001220051574 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Snowdon R. J. Institut für Pflanzenbau und Pflanzenzüchtung II, Justus-Liebig-Universität Giessen, IFZ, Heinrich-Buff-Ring 26, 35392 Giessen, Germany, DE aut NATIONALLICENCE 700 1- Winter H. Institut für Biologie - Angewandte Genetik, Freie Universität Berlin, Albrecht-Thaer-Weg 6, 14195 Berlin, Germany Fax: +49 (030) 838 54345 e-mail: sacris@zedat.fu-berlin.de, DE aut NATIONALLICENCE 700 1- Diestel A. Institut für Biologie - Angewandte Genetik, Freie Universität Berlin, Albrecht-Thaer-Weg 6, 14195 Berlin, Germany Fax: +49 (030) 838 54345 e-mail: sacris@zedat.fu-berlin.de, DE aut NATIONALLICENCE 700 1- Sacristán M. D. Institut für Biologie - Angewandte Genetik, Freie Universität Berlin, Albrecht-Thaer-Weg 6, 14195 Berlin, Germany Fax: +49 (030) 838 54345 e-mail: sacris@zedat.fu-berlin.de, DE aut Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer