caa a22 4500 475793625 CHVBK 20180406123717.0 cr unu---uuuuu 170329e20001101xx s 000 0 eng 10.1007/s001220051573 doi (NATIONALLICENCE)springer-10.1007/s001220051573 Development and applications of a set of chromosome-specific cytogenetic DNA markers in potato [Elektronische Daten] [F. Dong, J. Song, S. K. Naess, J. P. Helgeson, C. Gebhardt, J. Jiang] Abstract : Reliable and easy to use techniques for chromosome identification are critical for many aspects of cytogenetic research. Unfortunately, such techniques are not available in many plant species, especially those with a large number of small chromosomes. Here we demonstrate that fluorescence in situ hybridization (FISH) signals derived from bacterial artificial chromosomes (BACs) can be used as chromosome-specific cytogenetic DNA markers for chromosome identification in potato. We screened a potato BAC library using genetically mapped restriction fragment length polymorphism markers as probes. The identified BAC clones were then labeled as probes for FISH analysis. A set of 12 chromosome-specific BAC clones were isolated and the FISH signals derived from these BAC clones serve as convenient and reliable cytological markers for potato chromosome identification. We mapped the 5S rRNA genes, the 45S rRNA genes, and a potato late blight resistance gene to three specific potato chromosomes using the chromosome-specific BAC clones. Springer-Verlag Berlin Heidelberg, 2000 Key words Chromosome identification nationallicence Physical mapping nationallicence FISH nationallicence BAC nationallicence Molecular cytogenetics nationallicence Potato nationallicence Dong F. Department of Horticulture, University of Wisconsin-Madison, Madison, WI 53706, USA e-mail: jjiang1@facstaff.wisc.edu Fax: +1 608-262-4743, US aut Song J. Department of Horticulture, University of Wisconsin-Madison, Madison, WI 53706, USA e-mail: jjiang1@facstaff.wisc.edu Fax: +1 608-262-4743, US aut Naess S. K. USDA/ARS Plant Disease Resistance Research Unit, Department of Plant Pathology, University of Wisconsin-Madison, Madison, WI 53706, USA, US aut Helgeson J. P. USDA/ARS Plant Disease Resistance Research Unit, Department of Plant Pathology, University of Wisconsin-Madison, Madison, WI 53706, USA, US aut Gebhardt C. Max-Planck-Institut für Züchtungforschung, Carl-von-Linné-Weg 10, D-50829 Köln, Germany, DE aut Jiang J. Department of Horticulture, University of Wisconsin-Madison, Madison, WI 53706, USA e-mail: jjiang1@facstaff.wisc.edu Fax: +1 608-262-4743, US aut https://doi.org/10.1007/s001220051573 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/s001220051573 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Dong F. Department of Horticulture, University of Wisconsin-Madison, Madison, WI 53706, USA e-mail: jjiang1@facstaff.wisc.edu Fax: +1 608-262-4743, US aut NATIONALLICENCE 700 1- Song J. Department of Horticulture, University of Wisconsin-Madison, Madison, WI 53706, USA e-mail: jjiang1@facstaff.wisc.edu Fax: +1 608-262-4743, US aut NATIONALLICENCE 700 1- Naess S. K. USDA/ARS Plant Disease Resistance Research Unit, Department of Plant Pathology, University of Wisconsin-Madison, Madison, WI 53706, USA, US aut NATIONALLICENCE 700 1- Helgeson J. P. USDA/ARS Plant Disease Resistance Research Unit, Department of Plant Pathology, University of Wisconsin-Madison, Madison, WI 53706, USA, US aut NATIONALLICENCE 700 1- Gebhardt C. Max-Planck-Institut für Züchtungforschung, Carl-von-Linné-Weg 10, D-50829 Köln, Germany, DE aut NATIONALLICENCE 700 1- Jiang J. Department of Horticulture, University of Wisconsin-Madison, Madison, WI 53706, USA e-mail: jjiang1@facstaff.wisc.edu Fax: +1 608-262-4743, US aut Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer