caa a22 4500 475793773 CHVBK 20180406123717.0 cr unu---uuuuu 170329e20000801xx s 000 0 eng 10.1007/s001220051504 doi (NATIONALLICENCE)springer-10.1007/s001220051504 Characterization of sources of resistance to the watermelon strain of Papaya ringspot virus in cucumber: allelism and co-segregation with other potyvirus resistances [Elektronische Daten] [R. Grumet, E. Kabelka, S. McQueen, T. Wai, R. Humphrey] Abstract : At least three sources of resistance to the watermelon strain of Papaya ringspot virus (PRSV-W) have been identified in cucumber (Cucumis sativus L.) including: 'TMG-1', an inbred line derived from the Taiwanese cultivar, 'Taichung Mou Gua'; 'Dina-1', an inbred line derived from the Dutch hybrid 'Dina'; and the South American cultivar 'Surinam'. In this investigation we sought to determine the inheritance of resistance to PRSV-W in 'Dina-1', the allelic relationships among the three sources of PRSV-W resistance, and the relationship between PRSV-W resistance and known resistances to other cucurbit potyviruses. Like 'Surinam' and 'TMG-1', resistance in 'Dina-1' is controlled by a single gene. Despite differences in dominance vs recessive performance and patterns of virus accumulation, all three sources of resistance complemented each other. 'TMG-1' and 'Dina-1' also possess co-segregating, single-gene resistances to Zucchini yellow mosaic virus (ZYMV), Watermelon mosaic virus and Moroccan watermelon mosaic virus. Sequential inoculations and F3 family analysis indicated that resistance to PRSV-W completely co- segregated with resistance to ZYMV in 'TMG-1'. Although PRSV-W resistances are at the same locus in both 'TMG-1' and 'Surinam', 'Surinam' is only resistant to PRSV-W, and progeny of 'TMG-1'×'Surinam' were resistant to PRSV-W but susceptible to ZYMV. Susceptibility to ZYMV and resistance to PRSV-W in 'Surinam' was not influenced by co-inoculation or sequential in- oculations of the two viruses. Collectively, the co- segregation of resistances to PRSV-W, ZYMV, WMV and MWMV in 'TMG-1' (within 1 cM), allelism of PRSV-W resistances in 'TMG-1' and 'Surinam', and resistance to only PRSV-W in 'Surinam', suggest that multiple potyvirus resistance in cucumber may be due to different alleles of a single potyvirus resistance gene with differing viral specificities, or that the multiple resistances are conferred by a tightly linked cluster of resistance genes, of which 'Surinam' only possesses one member. Springer-Verlag Berlin Heidelberg, 2000 Key words Key words: Cucumis sativus nationallicence Resistance genes nationallicence Virus resistance nationallicence Gene cluster nationallicence Grumet R. Department of Horticulture, Michigan State University, East Lansing MI 48824, USA e-mail: grumet@pilot.msu.edu Tel.: +517-353-5568, US aut Kabelka E. Department of Horticulture, Michigan State University, East Lansing MI 48824, USA e-mail: grumet@pilot.msu.edu Tel.: +517-353-5568, US aut McQueen S. Department of Horticulture, Michigan State University, East Lansing MI 48824, USA e-mail: grumet@pilot.msu.edu Tel.: +517-353-5568, US aut Wai T. Department of Horticulture, Michigan State University, East Lansing MI 48824, USA e-mail: grumet@pilot.msu.edu Tel.: +517-353-5568, US aut Humphrey R. Department of Horticulture, Michigan State University, East Lansing MI 48824, USA e-mail: grumet@pilot.msu.edu Tel.: +517-353-5568, US aut https://doi.org/10.1007/s001220051504 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/s001220051504 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Grumet R. Department of Horticulture, Michigan State University, East Lansing MI 48824, USA e-mail: grumet@pilot.msu.edu Tel.: +517-353-5568, US aut NATIONALLICENCE 700 1- Kabelka E. Department of Horticulture, Michigan State University, East Lansing MI 48824, USA e-mail: grumet@pilot.msu.edu Tel.: +517-353-5568, US aut NATIONALLICENCE 700 1- McQueen S. Department of Horticulture, Michigan State University, East Lansing MI 48824, USA e-mail: grumet@pilot.msu.edu Tel.: +517-353-5568, US aut NATIONALLICENCE 700 1- Wai T. Department of Horticulture, Michigan State University, East Lansing MI 48824, USA e-mail: grumet@pilot.msu.edu Tel.: +517-353-5568, US aut NATIONALLICENCE 700 1- Humphrey R. Department of Horticulture, Michigan State University, East Lansing MI 48824, USA e-mail: grumet@pilot.msu.edu Tel.: +517-353-5568, US aut Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer