caa a22 4500 475793781 CHVBK 20180406123717.0 cr unu---uuuuu 170329e20000801xx s 000 0 eng 10.1007/s001220051506 doi (NATIONALLICENCE)springer-10.1007/s001220051506 Genetic analyses supported by molecular methods provide evidence of a new genic (st1) and a new cytoplasmic (st2) male sterility in Allium schoenoprasum L [Elektronische Daten] [T. Engelke, T. Tatlioglu] Abstract : Spontaneous mutations leading to male sterility have been described for many different crops and are of great importance to hybrid breeding, provided that their inheritance is resolved. This paper describes an efficient method to characterise male sterilities with respect to cytoplasmic factors that might be causally related to them. The differentiation of cytoplasmic (CMS) and genic (GMS) male sterility is achieved by a specific transfer of nuclear male sterility factors to different cytoplasm types which have previously been distinguished by means of RFLP analyses using mitochondrial gene probes. The nuclear sterility factors of Allium schoenoprasum used, st1 and st2, showed a monogenic recessive inheritance in their original cytoplasms. While st1 was expressed in four different cytoplasm types, st2 did not show itself in a cytoplasm type differing from the original. Therefore, the st1-sterility is a GMS, while a cytoplasmic factor is necessary for the occurrence of st2-sterility. This cytoplasmic factor was verified by a reciprocal cross, and the CMS system was completed by the selection of maintainer genotypes. Neither of these new sterilities were influenced by high temperatures or tetracycline. The benefits of a new CMS system to practical breeding and the advantages and disadvantages of the environmental influences on the expression of male sterility are discussed. Springer-Verlag Berlin Heidelberg, 2000 Key words Cytoplasmic male sterility nationallicence CMS nationallicence Genic male sterility nationallicence GMS nationallicence Chives nationallicence Mitochondrial genome nationallicence Engelke T. Abteilung Angewandte Genetik, Universität Hannover, Herrenhäuser Str. 2, D-30419 Hannover, Germany e-mail: thomas.engelke@mbox.genetik.uni-hannover.de Fax: +49-511-762-3608, DE aut Tatlioglu T. Abteilung Angewandte Genetik, Universität Hannover, Herrenhäuser Str. 2, D-30419 Hannover, Germany e-mail: thomas.engelke@mbox.genetik.uni-hannover.de Fax: +49-511-762-3608, DE aut https://doi.org/10.1007/s001220051506 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/s001220051506 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Engelke T. Abteilung Angewandte Genetik, Universität Hannover, Herrenhäuser Str. 2, D-30419 Hannover, Germany e-mail: thomas.engelke@mbox.genetik.uni-hannover.de Fax: +49-511-762-3608, DE aut NATIONALLICENCE 700 1- Tatlioglu T. Abteilung Angewandte Genetik, Universität Hannover, Herrenhäuser Str. 2, D-30419 Hannover, Germany e-mail: thomas.engelke@mbox.genetik.uni-hannover.de Fax: +49-511-762-3608, DE aut Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer