caa a22 4500 475793889 CHVBK 20180406123718.0 cr unu---uuuuu 170329e20001001xx s 000 0 eng 10.1007/s001220051540 doi (NATIONALLICENCE)springer-10.1007/s001220051540 Two large-insert soybean genomic libraries constructed in a binary vector: applications in chromosome walking and genome wide physical mapping [Elektronische Daten] [K. Meksem, K. Zobrist, E. Ruben, D. Hyten, T. Quanzhou, H-B. Zhang, D. A. Lightfoot] Abstract : Large DNA insert libraries in binary T-DNA vectors can assist in the isolation of the gene(s) underlying a quantitative trait locus (QTL). Binary vectors facilitate the transfer of large-insert DNA fragments containing a QTL from E. coli to Agrobacterium sp. and then to plants. We constructed two soybean large-insert libraries from cv. Forrest in the pCLD04541 (V41) binary vector after partial digestion of genomic high-molecular-weight DNA with BamHI or HindIII. The libraries contain 76,800 clones with an average insert size of 125 kb, and therefore represent 9.5-fold haploid genome equivalents. Colony hybridization using a chloroplast-specific probe infers that the libraries contain less than 0.5% clones of chloroplast DNA origin. These two libraries have provided clones for physical mapping of the soybean genome and for isolation of a number of disease resistance genes. One microsatellite marker was identified from the clone that hybridized to the Bng122 RFLP probe. The sequence-tagged site was used for genetic mapping and marker-assisted selection for genes underlying resistance to the soybean cyst nematode and sudden death syndrome. Springer-Verlag Berlin Heidelberg, 2000 Key words Bacterial artificial chromosome nationallicence Soybean nationallicence Physical mapping nationallicence Targeted microsatellites nationallicence Positional cloning nationallicence Disease resistance nationallicence Genomics nationallicence Cyst nematode nationallicence Meksem K. Department of Plant Soil and General Agriculture, Southern Illinois University at Carbondale, Room 176. Carbondale, IL 62901-4415, USA Fax: +618 453 7457 e-mail: http://www.coalab.siu.edu/genome/meksemk@siu.edu, US aut Zobrist K. Department of Plant Soil and General Agriculture, Southern Illinois University at Carbondale, Room 176. Carbondale, IL 62901-4415, USA Fax: +618 453 7457 e-mail: http://www.coalab.siu.edu/genome/meksemk@siu.edu, US aut Ruben E. Department of Plant Soil and General Agriculture, Southern Illinois University at Carbondale, Room 176. Carbondale, IL 62901-4415, USA Fax: +618 453 7457 e-mail: http://www.coalab.siu.edu/genome/meksemk@siu.edu, US aut Hyten D. Department of Plant Soil and General Agriculture, Southern Illinois University at Carbondale, Room 176. Carbondale, IL 62901-4415, USA Fax: +618 453 7457 e-mail: http://www.coalab.siu.edu/genome/meksemk@siu.edu, US aut Quanzhou T. Department of Soil and Crop Sciences, Crop Biotechnology Center, Texas A & M University, College Station, TX 77843, USA, US aut Zhang H-B Department of Soil and Crop Sciences, Crop Biotechnology Center, Texas A & M University, College Station, TX 77843, USA, US aut Lightfoot D. A. Department of Plant Soil and General Agriculture, Southern Illinois University at Carbondale, Room 176. Carbondale, IL 62901-4415, USA Fax: +618 453 7457 e-mail: http://www.coalab.siu.edu/genome/meksemk@siu.edu, US aut https://doi.org/10.1007/s001220051540 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/s001220051540 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Meksem K. Department of Plant Soil and General Agriculture, Southern Illinois University at Carbondale, Room 176. Carbondale, IL 62901-4415, USA Fax: +618 453 7457 e-mail: http://www.coalab.siu.edu/genome/meksemk@siu.edu, US aut NATIONALLICENCE 700 1- Zobrist K. Department of Plant Soil and General Agriculture, Southern Illinois University at Carbondale, Room 176. Carbondale, IL 62901-4415, USA Fax: +618 453 7457 e-mail: http://www.coalab.siu.edu/genome/meksemk@siu.edu, US aut NATIONALLICENCE 700 1- Ruben E. Department of Plant Soil and General Agriculture, Southern Illinois University at Carbondale, Room 176. Carbondale, IL 62901-4415, USA Fax: +618 453 7457 e-mail: http://www.coalab.siu.edu/genome/meksemk@siu.edu, US aut NATIONALLICENCE 700 1- Hyten D. Department of Plant Soil and General Agriculture, Southern Illinois University at Carbondale, Room 176. Carbondale, IL 62901-4415, USA Fax: +618 453 7457 e-mail: http://www.coalab.siu.edu/genome/meksemk@siu.edu, US aut NATIONALLICENCE 700 1- Quanzhou T. Department of Soil and Crop Sciences, Crop Biotechnology Center, Texas A & M University, College Station, TX 77843, USA, US aut NATIONALLICENCE 700 1- Zhang H-B Department of Soil and Crop Sciences, Crop Biotechnology Center, Texas A & M University, College Station, TX 77843, USA, US aut NATIONALLICENCE 700 1- Lightfoot D. A. Department of Plant Soil and General Agriculture, Southern Illinois University at Carbondale, Room 176. Carbondale, IL 62901-4415, USA Fax: +618 453 7457 e-mail: http://www.coalab.siu.edu/genome/meksemk@siu.edu, US aut Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer