caa a22 4500 475793919 CHVBK 20180406123718.0 cr unu---uuuuu 170329e20001001xx s 000 0 eng 10.1007/s001220051543 doi (NATIONALLICENCE)springer-10.1007/s001220051543 Havey M. J. United States Department of Agriculture, Agricultural Research Service, Department of Horticulture, 1575 Linden Drive, University of Wisconsin, Madison, WI 53706, USA, US aut Diversity among male-sterility-inducing and male-fertile cytoplasms of onion [Elektronische Daten] [M. J. Havey] Abstract : Hybrid-onion (Allium cepa) seed is produced using systems of cytoplasmic-genic male sterility (CMS). Two different sources of CMS (S and T cytoplasms) have been genetically characterized. Testcrosses of N-cytoplasmic maintaining and restoring genotypes to S and T cytoplasmic lines demonstrated that different alleles, or loci, restore male fertility for these two male-sterile cytoplasms. Other sources of CMS have been used or reported in Europe, Japan and India, and their relationships to S and T cytoplasms are not clear. Restriction fragment length polymorphisms were identified in the organellar genomes among commercially used male-sterile cytoplasms from Holland, Japan and India, and were compared to S and T cytoplasms. Mitochondrial DNA diversity among 58 non-S-cytoplasmic open-pollinated onion populations was also assessed. All five putative CMS lines selected from the Indian population Nasik White Globe were identical to S cytoplasm for all polymorphisms in the chloroplast genome, and always possessed the same-sized mitochondrial fragments as S cytoplasm. T cytoplasm, the male-sterile cytoplasm used to produce the Dutch hybrid Hygro F1, and two sources of CMS from Japan, were similar and showed numbers of mitochondrial polymorphisms similar to those observed among the 58 non-S-cytoplasmic open-pollinated populations. This research demonstrates that the same, or very similar, male-sterile cytoplasms have been independently isolated and exploited for hybrid-seed production in onion. Springer-Verlag Berlin Heidelberg, 2000 Key words Cytoplasmic male sterility nationallicence Nuclear male fertility restoration nationallicence Organellar DNA nationallicence Allium cepa nationallicence https://doi.org/10.1007/s001220051543 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/s001220051543 text/html Onlinezugriff via DOI NATIONALLICENCE 100 1- Havey M. J. United States Department of Agriculture, Agricultural Research Service, Department of Horticulture, 1575 Linden Drive, University of Wisconsin, Madison, WI 53706, USA, US aut Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer