caa a22 4500 475793935 CHVBK 20180406123718.0 cr unu---uuuuu 170329e20001001xx s 000 0 eng 10.1007/s001220051539 doi (NATIONALLICENCE)springer-10.1007/s001220051539 An allele of the 1-aminocyclopropane-1-carboxylate synthase gene ( Md-ACS1 ) accounts for the low level of ethylene production in climacteric fruits of some apple cultivars [Elektronische Daten] [T. Harada, T. Sunako, Y. Wakasa, J. Soejima, T. Satoh, M. Niizeki] Abstract : An allele of the apple ripening-specific 1- aminocyclopropane-1-carboxylate (ACC) synthase gene (Md-ACS1-1) has a 5′-flanking region possessing an inserted retroposon-like sequence. Apple species can be classified into three groups that are heterozygous or homozygous for the ACS1-1 and ACS1-2 alleles. We measured the internal ethylene concentration (IEC) in climacteric fruit of 35 apple cultivars with respect to genotype. Eleven ACS1-2 homozygous cultivars exhibited much lower IECs than cultivars homozygous or heterozygous for ACS1-1. Furthermore, F1 ACS1-2 homozygous progeny derived from crosses between heterozygous cultivars had fruit with a very low IEC. These results are in accord with previous data indicating the absence of transcription from ACS1-2 in a heterozygous cultivar. Since the low level of ACS1 mRNA in climacteric fruit was observed in several ACS1-2 homozygous cultivars, we conclude that the low level of ethylene production in some cultivars is caused by the mutated allele of ACS1, which is the main gene responsible for ethylene production during ripening. Springer-Verlag Berlin Heidelberg, 2000 Key words Malus×domestica Borkh nationallicence Ethylene nationallicence Ripening nationallicence ACC synthase gene nationallicence Storage nationallicence Harada T. Laboratory of Plant Breeding and Genetics, Faculty of Agriculture and Life Science, Hirosaki University, Bunkyo-cho 3, Hirosaki 036-8561, Japan e-mail: tharada@cc.hirosaki-u.ac.jp Fax: +81-172-39-3750, Present address: T. Sunako, Bioscience Center and Graduate School of Bioagricultural Sciences, Nagoya University, Chikusa, Nagoya 464-8601, Japan, JP aut Sunako T. Laboratory of Plant Breeding and Genetics, Faculty of Agriculture and Life Science, Hirosaki University, Bunkyo-cho 3, Hirosaki 036-8561, Japan e-mail: tharada@cc.hirosaki-u.ac.jp Fax: +81-172-39-3750, Present address: T. Sunako, Bioscience Center and Graduate School of Bioagricultural Sciences, Nagoya University, Chikusa, Nagoya 464-8601, Japan, JP aut Wakasa Y. Laboratory of Plant Breeding and Genetics, Faculty of Agriculture and Life Science, Hirosaki University, Bunkyo-cho 3, Hirosaki 036-8561, Japan e-mail: tharada@cc.hirosaki-u.ac.jp Fax: +81-172-39-3750, Present address: T. Sunako, Bioscience Center and Graduate School of Bioagricultural Sciences, Nagoya University, Chikusa, Nagoya 464-8601, Japan, JP aut Soejima J. Apple Research Center, National Institute of Fruit Tree Science, Shimokuriyagawa, Morioka 020-0123, Japan, JP aut Satoh T. Aomori Apple Experimental Station, Botandaira, Kuroishi 036-0332, Japan, JP aut Niizeki M. Laboratory of Plant Breeding and Genetics, Faculty of Agriculture and Life Science, Hirosaki University, Bunkyo-cho 3, Hirosaki 036-8561, Japan e-mail: tharada@cc.hirosaki-u.ac.jp Fax: +81-172-39-3750, Present address: T. Sunako, Bioscience Center and Graduate School of Bioagricultural Sciences, Nagoya University, Chikusa, Nagoya 464-8601, Japan, JP aut https://doi.org/10.1007/s001220051539 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/s001220051539 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Harada T. Laboratory of Plant Breeding and Genetics, Faculty of Agriculture and Life Science, Hirosaki University, Bunkyo-cho 3, Hirosaki 036-8561, Japan e-mail: tharada@cc.hirosaki-u.ac.jp Fax: +81-172-39-3750, Present address: T. Sunako, Bioscience Center and Graduate School of Bioagricultural Sciences, Nagoya University, Chikusa, Nagoya 464-8601, Japan, JP aut NATIONALLICENCE 700 1- Sunako T. Laboratory of Plant Breeding and Genetics, Faculty of Agriculture and Life Science, Hirosaki University, Bunkyo-cho 3, Hirosaki 036-8561, Japan e-mail: tharada@cc.hirosaki-u.ac.jp Fax: +81-172-39-3750, Present address: T. Sunako, Bioscience Center and Graduate School of Bioagricultural Sciences, Nagoya University, Chikusa, Nagoya 464-8601, Japan, JP aut NATIONALLICENCE 700 1- Wakasa Y. Laboratory of Plant Breeding and Genetics, Faculty of Agriculture and Life Science, Hirosaki University, Bunkyo-cho 3, Hirosaki 036-8561, Japan e-mail: tharada@cc.hirosaki-u.ac.jp Fax: +81-172-39-3750, Present address: T. Sunako, Bioscience Center and Graduate School of Bioagricultural Sciences, Nagoya University, Chikusa, Nagoya 464-8601, Japan, JP aut NATIONALLICENCE 700 1- Soejima J. Apple Research Center, National Institute of Fruit Tree Science, Shimokuriyagawa, Morioka 020-0123, Japan, JP aut NATIONALLICENCE 700 1- Satoh T. Aomori Apple Experimental Station, Botandaira, Kuroishi 036-0332, Japan, JP aut NATIONALLICENCE 700 1- Niizeki M. Laboratory of Plant Breeding and Genetics, Faculty of Agriculture and Life Science, Hirosaki University, Bunkyo-cho 3, Hirosaki 036-8561, Japan e-mail: tharada@cc.hirosaki-u.ac.jp Fax: +81-172-39-3750, Present address: T. Sunako, Bioscience Center and Graduate School of Bioagricultural Sciences, Nagoya University, Chikusa, Nagoya 464-8601, Japan, JP aut Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer