caa a22 4500 475793943 CHVBK 20180406123718.0 cr unu---uuuuu 170329e20001001xx s 000 0 eng 10.1007/s001220051571 doi (NATIONALLICENCE)springer-10.1007/s001220051571 Development of simple sequence repeats (SSRs) in olive tree ( Olea europaea L.) [Elektronische Daten] [P. Rallo, G. Dorado, A. Martín] Abstract : We report the development of microsatellites or simple sequence repeats (SSRs) in the olive tree (Olea europaea L.). Forty three positive clones obtained by the screening of a GA-enriched genomic library were sequenced and primers were designed for 13 microsatellite loci. Five primer pairs amplified polymorphic products of the expected size range. SSR polymorphism was explored in a set of 46 olive cultivars. A total of 26 alleles were detected for the five loci. Heterozygosity ranged from 0.46 to 0.71. Ninety one per cent of the cultivars had unique multilocus genotypes. Microsatellite segregation was studied in a complex population from a cross between the commercial cultivars 'Leccino' and 'Dolce Agogia'. Springer-Verlag Berlin Heidelberg, 2000 Key words Microsatellites nationallicence Variable number of tandem repeats (VNTRs) nationallicence Enriched library nationallicence Hexamer repeat nationallicence Null allele nationallicence Marker-assisted selection (MAS) nationallicence Rallo P. Instituto de Agricultura Sostenible-CSIC, Alameda del Obispo s/n., P.O. box 4084. 14080-Córdoba, Spain e-mail: ge2ramop@uco.es Fax: + 34-957 499252, ES aut Dorado G. Departamento de Bioquímica y Biología Molecular, Facultad de Veterinaria, Universidad de Córdoba, Spain, ES aut Martín A. Instituto de Agricultura Sostenible-CSIC, Alameda del Obispo s/n., P.O. box 4084. 14080-Córdoba, Spain e-mail: ge2ramop@uco.es Fax: + 34-957 499252, ES aut https://doi.org/10.1007/s001220051571 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/s001220051571 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Rallo P. Instituto de Agricultura Sostenible-CSIC, Alameda del Obispo s/n., P.O. box 4084. 14080-Córdoba, Spain e-mail: ge2ramop@uco.es Fax: + 34-957 499252, ES aut NATIONALLICENCE 700 1- Dorado G. Departamento de Bioquímica y Biología Molecular, Facultad de Veterinaria, Universidad de Córdoba, Spain, ES aut NATIONALLICENCE 700 1- Martín A. Instituto de Agricultura Sostenible-CSIC, Alameda del Obispo s/n., P.O. box 4084. 14080-Córdoba, Spain e-mail: ge2ramop@uco.es Fax: + 34-957 499252, ES aut Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer