caa a22 4500 475793951 CHVBK 20180406123718.0 cr unu---uuuuu 170329e20001001xx s 000 0 eng 10.1007/s001220051566 doi (NATIONALLICENCE)springer-10.1007/s001220051566 A high-density linkage map of Theobroma cacao L [Elektronische Daten] [A. M. Risterucci, L. Grivet, J. A. K. N'Goran, I. Pieretti, M. H. Flament, C. Lanaud] Abstract : The first linkage map established by Lanaud et al. (1995) was used as a starting point to produce a high-density molecular linkage map. A mapping population of 181 progenies resulting from a cross between two heterozygous genotypes, a Forastero and a Trinitario (hybrid between Forastero and Criollo), was used for the linkage analysis. A new DNA isolation protocol was established, which allows enough good quality DNA to construct a genetic map with PCR-based markers. The map comprises 424 markers with an average spacing between markers of 2.1 cM. The marker types used were five isozymes, six loci from known function genes, 65 genomic RFLPs, 104 cDNA RFLPs, three telomeric probes, 30 RAPDs, 191 AFLPs and 20 microsatellites. The use of new marker types, AFLP and microsatellites, did not disturb the original order of the RFLP loci used on the previous map. The genetic markers were distributed over ten linkage groups and cover 885.4 cM. The maximum distance observed between adjacent markers was 16.2 cM, and 9.4% of all loci showed skewed segregation. Springer-Verlag Berlin Heidelberg, 2000 Key words Theobroma cacao L nationallicence AFLP nationallicence Microsatellites nationallicence RFLP nationallicence High-density genetic map nationallicence Risterucci A. M. CIRAD, Centre de Cooperation Internationale en Recherche Agronomique pour le Développement, avenue Agropolis, BP 5035, 34032 Montpellier cedex, France e-mail: risterucci@cirad.fr Fax: +33467615605, FR aut Grivet L. CIRAD, Centre de Cooperation Internationale en Recherche Agronomique pour le Développement, avenue Agropolis, BP 5035, 34032 Montpellier cedex, France e-mail: risterucci@cirad.fr Fax: +33467615605, FR aut N'Goran J. A. K. IDEFOR-DCC, BP 1827, Abidjan 01, Co^te d'Ivoire aut Pieretti I. CIRAD, Centre de Cooperation Internationale en Recherche Agronomique pour le Développement, avenue Agropolis, BP 5035, 34032 Montpellier cedex, France e-mail: risterucci@cirad.fr Fax: +33467615605, FR aut Flament M. H. CIRAD, Centre de Cooperation Internationale en Recherche Agronomique pour le Développement, avenue Agropolis, BP 5035, 34032 Montpellier cedex, France e-mail: risterucci@cirad.fr Fax: +33467615605, FR aut Lanaud C. CIRAD, Centre de Cooperation Internationale en Recherche Agronomique pour le Développement, avenue Agropolis, BP 5035, 34032 Montpellier cedex, France e-mail: risterucci@cirad.fr Fax: +33467615605, FR aut https://doi.org/10.1007/s001220051566 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/s001220051566 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Risterucci A. M. CIRAD, Centre de Cooperation Internationale en Recherche Agronomique pour le Développement, avenue Agropolis, BP 5035, 34032 Montpellier cedex, France e-mail: risterucci@cirad.fr Fax: +33467615605, FR aut NATIONALLICENCE 700 1- Grivet L. CIRAD, Centre de Cooperation Internationale en Recherche Agronomique pour le Développement, avenue Agropolis, BP 5035, 34032 Montpellier cedex, France e-mail: risterucci@cirad.fr Fax: +33467615605, FR aut NATIONALLICENCE 700 1- N'Goran J. A. K. IDEFOR-DCC, BP 1827, Abidjan 01, Co^te d'Ivoire aut NATIONALLICENCE 700 1- Pieretti I. CIRAD, Centre de Cooperation Internationale en Recherche Agronomique pour le Développement, avenue Agropolis, BP 5035, 34032 Montpellier cedex, France e-mail: risterucci@cirad.fr Fax: +33467615605, FR aut NATIONALLICENCE 700 1- Flament M. H. CIRAD, Centre de Cooperation Internationale en Recherche Agronomique pour le Développement, avenue Agropolis, BP 5035, 34032 Montpellier cedex, France e-mail: risterucci@cirad.fr Fax: +33467615605, FR aut NATIONALLICENCE 700 1- Lanaud C. CIRAD, Centre de Cooperation Internationale en Recherche Agronomique pour le Développement, avenue Agropolis, BP 5035, 34032 Montpellier cedex, France e-mail: risterucci@cirad.fr Fax: +33467615605, FR aut Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer