caa a22 4500 475793978 CHVBK 20180406123718.0 cr unu---uuuuu 170329e20001001xx s 000 0 eng 10.1007/s001220051562 doi (NATIONALLICENCE)springer-10.1007/s001220051562 Development of diagnostic PCR markers closely linked to the tomato powdery mildew resistance gene Ol-1 on chromosome 6 of tomato [Elektronische Daten] [C. C. Huang, Y.-Y. Cui, C. R. Weng, P. Zabel, P. Lindhout] Abstract : Lycopersicon hirsutum G1.1560 is a wild accession of tomato that shows resistance to Oidium lycopersicum, a frequently occurring tomato powdery mildew. This resistance is largely controlled by an incompletely dominant gene Ol-1 near the Aps-1 locus in the vicinity of the resistance genes Mi and Cf-2/Cf-5. Using a new F2 population (n=150) segregating for resistance, we mapped the Ol-1 gene more accurately to a location between the RFLP markers TG153 and TG164. Furthermore, in saturating the Ol-1 region with more molecular markers using bulked segregant analysis, we were able to identify five RAPDs associated with the resistance. These RAPDs were then sequenced and converted into SCAR markers: SCAB01 and SCAF10 were L. hirsutum-specific; SCAE16, SCAG11 and SCAK16 were L. esculentum-specific. By linkage analysis a dense integrated map comprising RFLP and SCAR markers near Ol-1 was obtained. This will facilitate a map-based cloning approach for Ol-1 and marker-assisted selection for powdery mildew resistance in tomato breeding. Springer-Verlag Berlin Heidelberg, 2000 Keywords Resistance nationallicence Tomato powdery mildew nationallicence Tomato nationallicence Mapping nationallicence Oidium lycopersicum nationallicence RFLP nationallicence Sequence characterised amplified region (SCAR) nationallicence Huang C. C. Laboratory of Plant Breeding, The Graduate School of Experimental Plant Sciences, Wageningen Agricultural University, P.O. Box 386, 6700 AJ Wageningen, The Netherlands Fax: +31-317-483457, NL aut Cui Y.-Y Laboratory of Molecular Biology, The Graduate School of Experimental Plant Sciences, Wageningen, Agricultural University, P.O. Box 386, 6700 AJ Wageningen, The Netherlands, and the Department of Plant Pathology, College of Agriculture, Food & Natural resources, 108 Walters Hall, Plant Science Unit, University of Missouri-Columbia, Columbia, MO 65211, USA aut Weng C. R. Laboratory of Molecular Biology, The Graduate School of Experimental Plant Sciences, Wageningen Agricultural University, P.O. Box 386, 6700 AJ Wageningen, The Netherlands, NL aut Zabel P. Laboratory of Molecular Biology, The Graduate School of Experimental Plant Sciences, Wageningen Agricultural University, P.O. Box 386, 6700 AJ Wageningen, The Netherlands, NL aut Lindhout P. Laboratory of Plant Breeding, The Graduate School of Experimental Plant Sciences, Wageningen Agricultural University, P.O. Box 386, 6700 AJ Wageningen, The Netherlands Fax: +31-317-483457, NL aut https://doi.org/10.1007/s001220051562 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/s001220051562 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Huang C. C. Laboratory of Plant Breeding, The Graduate School of Experimental Plant Sciences, Wageningen Agricultural University, P.O. Box 386, 6700 AJ Wageningen, The Netherlands Fax: +31-317-483457, NL aut NATIONALLICENCE 700 1- Cui Y.-Y Laboratory of Molecular Biology, The Graduate School of Experimental Plant Sciences, Wageningen, Agricultural University, P.O. Box 386, 6700 AJ Wageningen, The Netherlands, and the Department of Plant Pathology, College of Agriculture, Food & Natural resources, 108 Walters Hall, Plant Science Unit, University of Missouri-Columbia, Columbia, MO 65211, USA aut NATIONALLICENCE 700 1- Weng C. R. Laboratory of Molecular Biology, The Graduate School of Experimental Plant Sciences, Wageningen Agricultural University, P.O. Box 386, 6700 AJ Wageningen, The Netherlands, NL aut NATIONALLICENCE 700 1- Zabel P. Laboratory of Molecular Biology, The Graduate School of Experimental Plant Sciences, Wageningen Agricultural University, P.O. Box 386, 6700 AJ Wageningen, The Netherlands, NL aut NATIONALLICENCE 700 1- Lindhout P. Laboratory of Plant Breeding, The Graduate School of Experimental Plant Sciences, Wageningen Agricultural University, P.O. Box 386, 6700 AJ Wageningen, The Netherlands Fax: +31-317-483457, NL aut Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer