caa a22 4500 475793986 CHVBK 20180406123718.0 cr unu---uuuuu 170329e20001001xx s 000 0 eng 10.1007/s001220051545 doi (NATIONALLICENCE)springer-10.1007/s001220051545 Expression of two soybean resistance gene candidates shows divergence of paralogous single-copy genes [Elektronische Daten] [A. J. Hayes, Y. G. Yue, M.A. Saghai Maroof] Abstract : The cloning of several plant genes directly involved in triggering a disease resistance response has shown that numerous resistance genes in the nucleotide binding site (NBS)/leucine-rich repeat (LRR) class have similar conserved amino acid sequences. In this study, we used a short soybean DNA sequence, previously cloned based on its conserved NBS, as a probe to identify full-length resistance gene candidates. Two homologous, but genetically independent genes were identified. One gene maps to the soybean molecular linkage group (MLG) F and a second is coded on MLG E. The first gene contains a 3,279 nucleotide open reading frame (ORF) sequence and possesses all the functional motifs characteristic of previously cloned NBS/LRR resistance genes. The N-terminal sequence of the deduced gene product is highly characteristic of other resistance genes in the subgroup of NBS/LRR genes which show homology to the Toll/Interleukin-1 receptor genes. The C-terminal region is somewhat more divergent as seen in other cloned disease resistance genes. This region of the F-linked gene contains an LRR region that is characterized by two alternatively spliced products which produce gene products with either a four-repeat or a ten-repeat LRR. The second cloned gene that maps to soybean MLG E contains 1,565 nucleotides of ORF in the N-terminal domain. Despite strong homology, however, the 3´ region of this gene contains several in-frame stop codons and apparent frame shifts compared to the F-linked gene, suggesting that its functionality as a disease resistance gene is questionable. These two disease resistance gene candidates are shown to be closely related to one another and to the members of the NBS/LRR class of disease resistance genes. Springer-Verlag Berlin Heidelberg, 2000 Key words Nucleotide binding site (NBS) nationallicence Leucine-rich repeat (LRR) nationallicence Disease resistance nationallicence Hayes A. J. Department of Crop and Soil Environmental Sciences, Virginia Tech, Blacksburg, VA 24061-0404, USA e-mail: smaroof@vt.edu, Fax: +1-540-231-3431, US aut Yue Y. G. Dow AgroSciences LLC, 9330 Zionsville Rd., Indianapolis, IN 46268, USA, US aut Saghai Maroof M.A. Department of Crop and Soil Environmental Sciences, Virginia Tech, Blacksburg, VA 24061-0404, USA e-mail: smaroof@vt.edu, Fax: +1-540-231-3431, US aut https://doi.org/10.1007/s001220051545 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/s001220051545 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Hayes A. J. Department of Crop and Soil Environmental Sciences, Virginia Tech, Blacksburg, VA 24061-0404, USA e-mail: smaroof@vt.edu, Fax: +1-540-231-3431, US aut NATIONALLICENCE 700 1- Yue Y. G. Dow AgroSciences LLC, 9330 Zionsville Rd., Indianapolis, IN 46268, USA, US aut NATIONALLICENCE 700 1- Saghai Maroof M.A. Department of Crop and Soil Environmental Sciences, Virginia Tech, Blacksburg, VA 24061-0404, USA e-mail: smaroof@vt.edu, Fax: +1-540-231-3431, US aut Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer