caa a22 4500 475793994 CHVBK 20180406123718.0 cr unu---uuuuu 170329e20001001xx s 000 0 eng 10.1007/s001220051535 doi (NATIONALLICENCE)springer-10.1007/s001220051535 Identification of different chromatin classes in wheat using in situ hybridization with simple sequence repeat oligonucleotides [Elektronische Daten] [A. Cuadrado, T. Schwarzacher, N. Jouve] Abstract : Clusters of four simple sequence repeats (SSRs), AAC, AAG, AG and CAT, have been mapped physically to hexaploid wheat chromosomes; 15—24-bp synthetic oligonucleotides were labelled by random-primer labelling and used as probes for fluorescent in situ hybridization with standard formamide and low-salt conditions. AAC hybridized strongly to the pericentromeric regions and several intercalary sites of all seven chromosomes of the B-genome corresponding to N bands and enabling their identification. Most of the AAC sites also co-localize with AAG, although the strength of the AAC and AAG signal was often different at the same location. Not all heterochromatic bands showed AAC signals and a few AAC sites were detected that are neither AAG nor N band positive, revealing the complex and heterogeneous genome organization of wheat and identifying the four most frequent classes of banded chromatin. Clusters characterised by a high concentration of AG repeats were detected on chromosome arms 3BS, 4BL, 5BS and 5BL, adjacent to AAG sites. The only detectable CAT cluster was found on chromosome arm 3BL, making this oligonucleotide valuable in identifying this particular chromosome. SSR in situ hybridization is useful as a diagnostic tool in cytogenetics and for understanding genome organization in wheat. Springer-Verlag Berlin Heidelberg, 2000 Key words Triticum aestivum L nationallicence Microsatellites nationallicence C banding nationallicence N banding nationallicence Genome organization nationallicence Cuadrado A. Department of Cell Biology and Genetics, University of Alcala, 28871 Alcala de Henares, Madrid, Spain e-mail: bcjouve@gene.alcala.es Tel.: +34-91-885 4750, Fax: +34-91-885 4799, ES aut Schwarzacher T. John Innes Centre, Norwich Research Park, Colney, Norwich NR4 7UH, UK, GB aut Jouve N. Department of Cell Biology and Genetics, University of Alcala, 28871 Alcala de Henares, Madrid, Spain e-mail: bcjouve@gene.alcala.es Tel.: +34-91-885 4750, Fax: +34-91-885 4799, ES aut https://doi.org/10.1007/s001220051535 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/s001220051535 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Cuadrado A. Department of Cell Biology and Genetics, University of Alcala, 28871 Alcala de Henares, Madrid, Spain e-mail: bcjouve@gene.alcala.es Tel.: +34-91-885 4750, Fax: +34-91-885 4799, ES aut NATIONALLICENCE 700 1- Schwarzacher T. John Innes Centre, Norwich Research Park, Colney, Norwich NR4 7UH, UK, GB aut NATIONALLICENCE 700 1- Jouve N. Department of Cell Biology and Genetics, University of Alcala, 28871 Alcala de Henares, Madrid, Spain e-mail: bcjouve@gene.alcala.es Tel.: +34-91-885 4750, Fax: +34-91-885 4799, ES aut Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer