caa a22 4500 47579401X CHVBK 20180406123718.0 cr unu---uuuuu 170329e20001001xx s 000 0 eng 10.1007/s001220051558 doi (NATIONALLICENCE)springer-10.1007/s001220051558 Ahmad M. New Zealand Institute for Crop & Food Research Limited, P.O. Box 4704, Christchurch, New Zealand e-mail: Ahmadm@crop.cri.nz, NZ aut Molecular marker-assisted selection of HMW glutenin alleles related to wheat bread quality by PCR-generated DNA markers [Elektronische Daten] [M. Ahmad] Abstract : While quality in hexaploid wheat (Triticum aestivum L. em Thell.) is a very complex trait, it is known that the water-insoluble gluten proteins are responsible for the elasticity and chohesiveness (strength) of dough and are therefore important determinants of breadmaking quality. High-molecular-weight (HMW) glutenin subunits encoded by genes on the long arm of group 1 chromosomes have been associated with gluten strength, and a portion of the variability between cultivars can be attributed to glutenin subunit composition. Good or poor wheat breadmaking quality is associated with two allelic pairs at the Glu-D1 complex locus, designated 1Dx5-1Dy10 and 1Dx2-1Dy12, respectively. Among the HMW glutenin subunits encoded at Glu-B1, Bx7 is quite common, being associated with either of two subunits, By8 or By9. Both allelic pairs contribute moderately well to good breadmaking quality by increasing dough elasticity. Glutenin subunit screening is accomplished using electrophoresis (SDS-PAGE). In this paper, I report the development of an alternative screening method based on glutenin genes themselves using the polymerase chain reaction (PCR). This easy, quick and non-destructive PCR-based approach is an efficient alternative to standard procedures for selecting bread-wheat genotypes with good breadmaking characteristics. Springer-Verlag Berlin Heidelberg, 2000 Key words Marker-assisted selection nationallicence PCR nationallicence Glutenin subunits nationallicence Bread quality nationallicence Hexaploid wheat nationallicence https://doi.org/10.1007/s001220051558 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/s001220051558 text/html Onlinezugriff via DOI NATIONALLICENCE 100 1- Ahmad M. New Zealand Institute for Crop & Food Research Limited, P.O. Box 4704, Christchurch, New Zealand e-mail: Ahmadm@crop.cri.nz, NZ aut Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer