caa a22 4500 475794036 CHVBK 20180406123718.0 cr unu---uuuuu 170329e20001001xx s 000 0 eng 10.1007/s001220051570 doi (NATIONALLICENCE)springer-10.1007/s001220051570 Identification of molecular markers linked to Rdr1 , a gene conferring resistance to blackspot in roses [Elektronische Daten] [B. von Malek, W. E Weber, T. Debener] Abstract : Blackspot resistance in the tetraploid rose genotype 91/100-5 had been characterised previously as a single dominant gene in duplex configuration. In the present study a tetraploid progeny (95/3) segregating for the presence of the blackspot resistance gene Rdr1 were screened with 868 RAPD and 114 AFLP primers/primer combinations. Seven AFLP markers were found to be linked to Rdr1 at distances between 1.1 and 7.6 cM. The most closely linked AFLP marker was cloned and converted into a SCAR marker that could be screened in a larger population than the original AFLP and was linked at a distance of 0.76 cM. The cloned fragment was used as an RFLP probe to locate the marker on a chromosome map of diploid roses. This is the first report of markers linked to a resistance gene in roses, and the possibilities of using them for a marker-assisted selection for blackspot resistance as well as for map-based cloning approaches are discussed. Springer-Verlag Berlin Heidelberg, 2000 Key words Blackspot nationallicence Diplocarpon nationallicence Molecular marker nationallicence Resistance gene nationallicence Rosa nationallicence von Malek B. Institute for Ornamental Plant Breeding, Bornkampsweg 31, D-22926 Ahrensburg, Germany Fax: +49-4102—51124 e-mail: tdebener@rrz.uni-hamburg.de, DE aut Weber W. E. Martin-Luther-Universität Halle-Wittenberg, Institut für Pflanzenzüchtung und Pflanzenschutz, D-06188 Hohenthurm, Germany, DE aut Debener T. Institute for Ornamental Plant Breeding, Bornkampsweg 31, D-22926 Ahrensburg, Germany Fax: +49-4102—51124 e-mail: tdebener@rrz.uni-hamburg.de, DE aut https://doi.org/10.1007/s001220051570 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/s001220051570 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- von Malek B. Institute for Ornamental Plant Breeding, Bornkampsweg 31, D-22926 Ahrensburg, Germany Fax: +49-4102—51124 e-mail: tdebener@rrz.uni-hamburg.de, DE aut NATIONALLICENCE 700 1- Weber W. E. Martin-Luther-Universität Halle-Wittenberg, Institut für Pflanzenzüchtung und Pflanzenschutz, D-06188 Hohenthurm, Germany, DE aut NATIONALLICENCE 700 1- Debener T. Institute for Ornamental Plant Breeding, Bornkampsweg 31, D-22926 Ahrensburg, Germany Fax: +49-4102—51124 e-mail: tdebener@rrz.uni-hamburg.de, DE aut Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer