caa a22 4500 475794079 CHVBK 20180406123718.0 cr unu---uuuuu 170329e20001001xx s 000 0 eng 10.1007/s001220051568 doi (NATIONALLICENCE)springer-10.1007/s001220051568 Application of synteny across Poaceae to determine the map location of a sugarcane rust resistance gene [Elektronische Daten] [C. Asnaghi, F. Paulet, C. Kaye, L. Grivet, M. Deu, J. C. Glaszmann, A. D'Hont] Abstract : A major rust resistance gene has been identified in a self-progeny of the sugarcane cultivar R570. Until now, this gene was known to be linked to a marker revealed by the sugarcane probe CDSR29 but unassigned to any linkage group of the current genetic map. We used synteny relationships between sugarcane and three other grasses in an attempt to saturate the region around this rust resistance gene. Comparison of sugarcane, sorghum, maize and rice genetic maps led to the identification of homoeologous chromosome segments at the extremity of sorghum linkage group D, rice linkage group 2, maize linkage group 4 and in the centromeric region of maize linkage group 5. One hundred and eighty-four heterologous probes were selected and tested for cross-hybridization with sugarcane DNA; 106 produced a good hybridization signal and were hybridized on 88 individuals of the R570 selfed progeny. Two hundred and seventeen single-dose markers were added to the R570 genetic map, of which 66% mapped to linkage group VII, together with the rust resistance gene. This gene has now been mapped to the end of a co-segregating group consisting of 19 RFLP markers. None of the mapped loci were located closer to the gene than CDSR29. The gene thus appears to reside at the edge of a ''synteny cluster'' used to describe the different grass genomes. Springer-Verlag Berlin Heidelberg, 2000 Key words Sugarcane nationallicence Rust resistance gene nationallicence Comparative mapping nationallicence RFLP nationallicence Asnaghi C. Centre de Coopération Internationale en Recherche Agronomique pour le Développement, Cirad, avenue Agropolis, 34398 Montpellier Cedex 5, France Fax: +33 4-67-61-56-05 e-mail: dhont@cirad.fr, FR aut Paulet F. Centre de Coopération Internationale en Recherche Agronomique pour le Développement, Cirad, avenue Agropolis, 34398 Montpellier Cedex 5, France Fax: +33 4-67-61-56-05 e-mail: dhont@cirad.fr, FR aut Kaye C. Centre de Coopération Internationale en Recherche Agronomique pour le Développement, Cirad, avenue Agropolis, 34398 Montpellier Cedex 5, France Fax: +33 4-67-61-56-05 e-mail: dhont@cirad.fr, FR aut Grivet L. Centre de Coopération Internationale en Recherche Agronomique pour le Développement, Cirad, avenue Agropolis, 34398 Montpellier Cedex 5, France Fax: +33 4-67-61-56-05 e-mail: dhont@cirad.fr, FR aut Deu M. Centre de Coopération Internationale en Recherche Agronomique pour le Développement, Cirad, avenue Agropolis, 34398 Montpellier Cedex 5, France Fax: +33 4-67-61-56-05 e-mail: dhont@cirad.fr, FR aut Glaszmann J. C. Centre de Coopération Internationale en Recherche Agronomique pour le Développement, Cirad, avenue Agropolis, 34398 Montpellier Cedex 5, France Fax: +33 4-67-61-56-05 e-mail: dhont@cirad.fr, FR aut D'Hont A. Centre de Coopération Internationale en Recherche Agronomique pour le Développement, Cirad, avenue Agropolis, 34398 Montpellier Cedex 5, France Fax: +33 4-67-61-56-05 e-mail: dhont@cirad.fr, FR aut https://doi.org/10.1007/s001220051568 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/s001220051568 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Asnaghi C. Centre de Coopération Internationale en Recherche Agronomique pour le Développement, Cirad, avenue Agropolis, 34398 Montpellier Cedex 5, France Fax: +33 4-67-61-56-05 e-mail: dhont@cirad.fr, FR aut NATIONALLICENCE 700 1- Paulet F. Centre de Coopération Internationale en Recherche Agronomique pour le Développement, Cirad, avenue Agropolis, 34398 Montpellier Cedex 5, France Fax: +33 4-67-61-56-05 e-mail: dhont@cirad.fr, FR aut NATIONALLICENCE 700 1- Kaye C. Centre de Coopération Internationale en Recherche Agronomique pour le Développement, Cirad, avenue Agropolis, 34398 Montpellier Cedex 5, France Fax: +33 4-67-61-56-05 e-mail: dhont@cirad.fr, FR aut NATIONALLICENCE 700 1- Grivet L. Centre de Coopération Internationale en Recherche Agronomique pour le Développement, Cirad, avenue Agropolis, 34398 Montpellier Cedex 5, France Fax: +33 4-67-61-56-05 e-mail: dhont@cirad.fr, FR aut NATIONALLICENCE 700 1- Deu M. Centre de Coopération Internationale en Recherche Agronomique pour le Développement, Cirad, avenue Agropolis, 34398 Montpellier Cedex 5, France Fax: +33 4-67-61-56-05 e-mail: dhont@cirad.fr, FR aut NATIONALLICENCE 700 1- Glaszmann J. C. Centre de Coopération Internationale en Recherche Agronomique pour le Développement, Cirad, avenue Agropolis, 34398 Montpellier Cedex 5, France Fax: +33 4-67-61-56-05 e-mail: dhont@cirad.fr, FR aut NATIONALLICENCE 700 1- D'Hont A. Centre de Coopération Internationale en Recherche Agronomique pour le Développement, Cirad, avenue Agropolis, 34398 Montpellier Cedex 5, France Fax: +33 4-67-61-56-05 e-mail: dhont@cirad.fr, FR aut Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer