caa a22 4500 475794087 CHVBK 20180406123718.0 cr unu---uuuuu 170329e20001001xx s 000 0 eng 10.1007/s001220051544 doi (NATIONALLICENCE)springer-10.1007/s001220051544 Molecular mapping of the Rph7.g leaf rust resistance gene in barley ( Hordeum vulgare L.) [Elektronische Daten] [S. Brunner, B. Keller, C. Feuillet] Abstract : In many temperate areas of the world, leaf rust is becoming an important disease of barley. In the last decade, new races of Puccinia hordei G. Otth have emerged which are virulent against the so-far most-effective race-specific resistance genes, such as Rph7. Marker-assisted selection greatly facilitates the pyramidization of two or more resistance genes in a single variety in order to achieve a more comprehensive resistance. Such a strategy requires the development of efficient and reliable markers. Here, we have developed a linkage map and found RFLP markers closely linked to the Rph7.g resistance gene on chromosome 3HS of barley. The receptor-like kinase gene Hv3Lrk that maps at 3.2 cM from Rph7.g was used to develop a PCR-based marker by exploiting a single nucleotide polymorphism. This marker was detected in 11 out of 12 (92%) barley lines having Rph7 and represents a valuable tool for marker-assisted selection. In addition, the identification of markers flanking Rph7.g provides the basis for positional cloning of this gene. Springer-Verlag Berlin Heidelberg, 2000 Key words Barley nationallicence Leaf rust nationallicence Marker-assisted selection nationallicence Resistance gene nationallicence Single-nucleotide polymorphism nationallicence Brunner S. Institut of Plant Biology, University of Zürich, Zollikerstrasse 107, CH-8008 Zürich, Switzerland e-mail: feuillet@botinst.unizh.ch Tel.: +41-16348231, Fax: +41-16348204, CH aut Keller B. Institut of Plant Biology, University of Zürich, Zollikerstrasse 107, CH-8008 Zürich, Switzerland e-mail: feuillet@botinst.unizh.ch Tel.: +41-16348231, Fax: +41-16348204, CH aut Feuillet C. Institut of Plant Biology, University of Zürich, Zollikerstrasse 107, CH-8008 Zürich, Switzerland e-mail: feuillet@botinst.unizh.ch Tel.: +41-16348231, Fax: +41-16348204, CH aut https://doi.org/10.1007/s001220051544 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/s001220051544 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Brunner S. Institut of Plant Biology, University of Zürich, Zollikerstrasse 107, CH-8008 Zürich, Switzerland e-mail: feuillet@botinst.unizh.ch Tel.: +41-16348231, Fax: +41-16348204, CH aut NATIONALLICENCE 700 1- Keller B. Institut of Plant Biology, University of Zürich, Zollikerstrasse 107, CH-8008 Zürich, Switzerland e-mail: feuillet@botinst.unizh.ch Tel.: +41-16348231, Fax: +41-16348204, CH aut NATIONALLICENCE 700 1- Feuillet C. Institut of Plant Biology, University of Zürich, Zollikerstrasse 107, CH-8008 Zürich, Switzerland e-mail: feuillet@botinst.unizh.ch Tel.: +41-16348231, Fax: +41-16348204, CH aut Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer