caa a22 4500 475794168 CHVBK 20180406123719.0 cr unu---uuuuu 170329e20001001xx s 000 0 eng 10.1007/s001220051572 doi (NATIONALLICENCE)springer-10.1007/s001220051572 Synthesis, morphology and cytogenetics of Raphanofortii (TTRR, 2n [Elektronische Daten] 38): a new amphidiploid of hybrid Brassica tournefortii (TT, 2n = 20) × Raphanus caudatus (RR, 2n=18) [B. R. Choudhary, P. Joshi, K. Singh] Abstract : Amphidiploid Raphanofortii was synthesized by colchicinization of the F1 hybrid Brassica tournefortii (TT, 2n = 20)×Raphanus caudatus (RR, 2n = 18). The crossability between these two species, and the cytomorphology of the F1 plants and the amphidiploids were investigated. Intergeneric hybrids between the species were obtained only when B. tournefortii was involved as female parent. The hybrid plants were intermediate for most of the morphological attributes and showed very low pollen fertility compared to the parents. Although a majority of the pollen mother cells of the dihaploid hybrid (TR, 2n = 19) harboured univalents, a maximum of six bivalents were also observed. Of the 37 colchicine-treated F1 plants analyzed cytologically, 21 were found to be true amphidiploids (2n = 38), whereas seven were mixoploids. Meiosis in the amphidiploids was characterized by the occurrence of 19 bivalents, though multivalents and univalents were also observed in a few cells. Most of the amphidiploid plants exhibited a fairly high pollen and seed fertility, which was further enhanced with the advancement of generations. Out of 69 plants investigated in the A2 generation, 64 were euploids while the remaining five were aneuploids (2n = 36, 37, 39, 40 and 42). The newly synthesized Raphanofortii has great potential as a new commercial crop, as well as a bridge species for the transfer of economically important attributes of both the species to other Brassicas. Springer-Verlag Berlin Heidelberg, 2000 Key words Brassica nationallicence Raphanus nationallicence Intergeneric hybrids nationallicence Amphidiploid nationallicence Raphanofortii nationallicence Morphology nationallicence Cytogenetics nationallicence Choudhary B. R. Agricultural Research Station, Rajasthan Agricutural University, Mandor-Jodhpur 342 304, India, IN aut Joshi P. Agricultural Research Station, Rajasthan Agricutural University, Mandor-Jodhpur 342 304, India, IN aut Singh K. Agricultural Research Station, Rajasthan Agricutural University, Mandor-Jodhpur 342 304, India, IN aut https://doi.org/10.1007/s001220051572 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/s001220051572 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Choudhary B. R. Agricultural Research Station, Rajasthan Agricutural University, Mandor-Jodhpur 342 304, India, IN aut NATIONALLICENCE 700 1- Joshi P. Agricultural Research Station, Rajasthan Agricutural University, Mandor-Jodhpur 342 304, India, IN aut NATIONALLICENCE 700 1- Singh K. Agricultural Research Station, Rajasthan Agricutural University, Mandor-Jodhpur 342 304, India, IN aut Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer