caa a22 4500 475794206 CHVBK 20180406123719.0 cr unu---uuuuu 170329e20001001xx s 000 0 eng 10.1007/s001220051546 doi (NATIONALLICENCE)springer-10.1007/s001220051546 Accumulation of maize γ-zein and γ-zein: KDEL to high levels in tobacco leaves and differential increase of BiP synthesis in transformants [Elektronische Daten] [M. Bellucci, A. Alpini, F. Paolocci, L. Cong, S. Arcioni] Abstract : Two gene constructs (pROK.TG1L and pROK.TG1LK) were utilized to achieve accumulation of maize γ-zein to high levels in tobacco (Nicotiana tabacum L.) leaves. Both the chimaeric genes contained the γ-zein-coding region preceded by the 5′untranslated leader from the coat protein mRNA of TMV, but one of them (pROK.TG1LK) was modified in its protein-coding region by the addition of the ER retention signal KDEL. The accumulation of γ-zein and γ-zein:KDEL in leaves was compared with heterologous protein accumulation in tobacco plants previously transformed with a γ-zein cDNA harbouring a native 5′UTR. Replacement of γ-zein 5′UTR with the TMV leader dramatically increased γ-zein production. Furthermore, γ-zein:KDEL-expressing plants, on average, accumulated twice as much foreign protein in their leaves as pROK.TG1L plants. The two-fold increase in the level of γ-zein:KDEL can probably be attributed to an improvement in the mechanism for ER retention of zeins in the transgenic cells. Transformants also showed increased production of BiP, though to a lesser extent in γ-zein:KDEL-expressing plants compared with pROK.TG1L plants. It is therefore likely that γ-zein:KDEL retention is made less dependent on the chaperone assistance of BiP by the presence of the KDEL signal on the γ-zein mutant. Springer-Verlag Berlin Heidelberg, 2000 Key words Gene expression nationallicence KDEL nationallicence Nicotiana nationallicence Transgenic plants nationallicence γ-Zein nationallicence Bellucci M. Istituto di Ricerche sul Miglioramento Genetico delle Piante Foraggere, CNR, via Madonna Alta 130, 06128, Perugia, Italy e-mail: s.arcioni@irmgpf.pg.cnr.it Tel.: +39-075-5005217, Fax: +39-075-5005228, Present address: L. Cong, Institute of Animal Science, CAAS, Yuanming Yuan West Road 2, Haidian, 100094, Beijing, China, IT aut Alpini A. Istituto di Ricerche sul Miglioramento Genetico delle Piante Foraggere, CNR, via Madonna Alta 130, 06128, Perugia, Italy e-mail: s.arcioni@irmgpf.pg.cnr.it Tel.: +39-075-5005217, Fax: +39-075-5005228, Present address: L. Cong, Institute of Animal Science, CAAS, Yuanming Yuan West Road 2, Haidian, 100094, Beijing, China, IT aut Paolocci F. Istituto di Ricerche sul Miglioramento Genetico delle Piante Foraggere, CNR, via Madonna Alta 130, 06128, Perugia, Italy e-mail: s.arcioni@irmgpf.pg.cnr.it Tel.: +39-075-5005217, Fax: +39-075-5005228, Present address: L. Cong, Institute of Animal Science, CAAS, Yuanming Yuan West Road 2, Haidian, 100094, Beijing, China, IT aut Cong L. Istituto di Ricerche sul Miglioramento Genetico delle Piante Foraggere, CNR, via Madonna Alta 130, 06128, Perugia, Italy e-mail: s.arcioni@irmgpf.pg.cnr.it Tel.: +39-075-5005217, Fax: +39-075-5005228, Present address: L. Cong, Institute of Animal Science, CAAS, Yuanming Yuan West Road 2, Haidian, 100094, Beijing, China, IT aut Arcioni S. Istituto di Ricerche sul Miglioramento Genetico delle Piante Foraggere, CNR, via Madonna Alta 130, 06128, Perugia, Italy e-mail: s.arcioni@irmgpf.pg.cnr.it Tel.: +39-075-5005217, Fax: +39-075-5005228, Present address: L. Cong, Institute of Animal Science, CAAS, Yuanming Yuan West Road 2, Haidian, 100094, Beijing, China, IT aut https://doi.org/10.1007/s001220051546 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/s001220051546 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Bellucci M. Istituto di Ricerche sul Miglioramento Genetico delle Piante Foraggere, CNR, via Madonna Alta 130, 06128, Perugia, Italy e-mail: s.arcioni@irmgpf.pg.cnr.it Tel.: +39-075-5005217, Fax: +39-075-5005228, Present address: L. Cong, Institute of Animal Science, CAAS, Yuanming Yuan West Road 2, Haidian, 100094, Beijing, China, IT aut NATIONALLICENCE 700 1- Alpini A. Istituto di Ricerche sul Miglioramento Genetico delle Piante Foraggere, CNR, via Madonna Alta 130, 06128, Perugia, Italy e-mail: s.arcioni@irmgpf.pg.cnr.it Tel.: +39-075-5005217, Fax: +39-075-5005228, Present address: L. Cong, Institute of Animal Science, CAAS, Yuanming Yuan West Road 2, Haidian, 100094, Beijing, China, IT aut NATIONALLICENCE 700 1- Paolocci F. Istituto di Ricerche sul Miglioramento Genetico delle Piante Foraggere, CNR, via Madonna Alta 130, 06128, Perugia, Italy e-mail: s.arcioni@irmgpf.pg.cnr.it Tel.: +39-075-5005217, Fax: +39-075-5005228, Present address: L. Cong, Institute of Animal Science, CAAS, Yuanming Yuan West Road 2, Haidian, 100094, Beijing, China, IT aut NATIONALLICENCE 700 1- Cong L. Istituto di Ricerche sul Miglioramento Genetico delle Piante Foraggere, CNR, via Madonna Alta 130, 06128, Perugia, Italy e-mail: s.arcioni@irmgpf.pg.cnr.it Tel.: +39-075-5005217, Fax: +39-075-5005228, Present address: L. Cong, Institute of Animal Science, CAAS, Yuanming Yuan West Road 2, Haidian, 100094, Beijing, China, IT aut NATIONALLICENCE 700 1- Arcioni S. Istituto di Ricerche sul Miglioramento Genetico delle Piante Foraggere, CNR, via Madonna Alta 130, 06128, Perugia, Italy e-mail: s.arcioni@irmgpf.pg.cnr.it Tel.: +39-075-5005217, Fax: +39-075-5005228, Present address: L. Cong, Institute of Animal Science, CAAS, Yuanming Yuan West Road 2, Haidian, 100094, Beijing, China, IT aut Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer