caa a22 4500 475794222 CHVBK 20180406123719.0 cr unu---uuuuu 170329e20001001xx s 000 0 eng 10.1007/s001220051556 doi (NATIONALLICENCE)springer-10.1007/s001220051556 High-molecular-weight glutenin subunits in the Cylindropyrum and Vertebrata section of the Aegilops genus and identification of subunits related to those encoded by the Dx alleles of common wheat [Elektronische Daten] [Y. Wan, K. Liu, D. Wang, P. R. Shewry] Abstract : The high-molecular-weight (HMW) glute-nin subunit composition of seven species from the Cylindropyrum and Vertebrata sections of the Aegilops genus was studied using SDS-PAGE and Western blot analysis. Two subunits were detected in Ae. caudata and three in Ae. cylindrica. In both species, subunits showing electrophoretic mobility similar to that of 1Dx2 were present. Western blot analysis using a monoclonal antibody (IFRN 1602) specific for the 1Ax and 1Dx subunits of bread wheat showed that the 1Dx-like subunit of Ae. caudata gave only a weak reaction. This indicates that Ae. caudata expresses subunits which are more distantly related to the 1Dx subunits. Two subunits were detected in each of the 60 accessions of Ae. tauschii, including several 1Dtx subunits showing different electrophoretic mobilities from those of the 1Dx subunits commonly found in bread wheat. All of the 1Dtx subunits reacted strongly with IFRN 1602, confirming their close relationship to the 1Dx subunits of bread wheat. Three subunits were found in Ae. crassa (6 x), four in Ae. ventricosa and Ae. juvenalis and five in Ae. vavilovii. In these four species, the subunits that showed electrophoretic mobility similar, or close, to that of 1Dx2 all reacted with IFRN 1602. In addition, Ae. ventricosa contained a subunit showing electrophoretic mobility slower than that of 1Dx2.2, which also reacted with IFRN 1602. These results suggest that the D-genome component in the multiploid Aegilops species express at least one HMW glutenin subunit that is structurally related to the 1Dx subunits of bread wheat. Springer-Verlag Berlin Heidelberg, 2000 Key words HMW glutenins nationallicence Aegilops tauschii nationallicence Cylindropyrum nationallicence Vertebrata nationallicence Wan Y. The National Key Laboratory for Plant Cell and Chromosome Engineering, Institute of Genetics, The Chinese Academy of Sciences, Beijing 100101, China e-mail: dwwang@igtp.ac.cn Fax: 86-10-64873482, CN aut Liu K. The National Key Laboratory for Plant Cell and Chromosome Engineering, Institute of Genetics, The Chinese Academy of Sciences, Beijing 100101, China e-mail: dwwang@igtp.ac.cn Fax: 86-10-64873482, CN aut Wang D. The National Key Laboratory for Plant Cell and Chromosome Engineering, Institute of Genetics, The Chinese Academy of Sciences, Beijing 100101, China e-mail: dwwang@igtp.ac.cn Fax: 86-10-64873482, CN aut Shewry P. R. IACR-Long Ashton Research Station, Department of Agricultural Sciences, University of Bristol, Long Ashton, Bristol BS18 9AF, UK, GB aut https://doi.org/10.1007/s001220051556 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/s001220051556 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Wan Y. The National Key Laboratory for Plant Cell and Chromosome Engineering, Institute of Genetics, The Chinese Academy of Sciences, Beijing 100101, China e-mail: dwwang@igtp.ac.cn Fax: 86-10-64873482, CN aut NATIONALLICENCE 700 1- Liu K. The National Key Laboratory for Plant Cell and Chromosome Engineering, Institute of Genetics, The Chinese Academy of Sciences, Beijing 100101, China e-mail: dwwang@igtp.ac.cn Fax: 86-10-64873482, CN aut NATIONALLICENCE 700 1- Wang D. The National Key Laboratory for Plant Cell and Chromosome Engineering, Institute of Genetics, The Chinese Academy of Sciences, Beijing 100101, China e-mail: dwwang@igtp.ac.cn Fax: 86-10-64873482, CN aut NATIONALLICENCE 700 1- Shewry P. R. IACR-Long Ashton Research Station, Department of Agricultural Sciences, University of Bristol, Long Ashton, Bristol BS18 9AF, UK, GB aut Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer