caa a22 4500 475794249 CHVBK 20180406123719.0 cr unu---uuuuu 170329e20001001xx s 000 0 eng 10.1007/s001220051564 doi (NATIONALLICENCE)springer-10.1007/s001220051564 Molecular phylogeny of Gossypium species by DNA fingerprinting [Elektronische Daten] [S. A. Khan, D. Hussain, E. Askari, J. McD Stewart, K. A. Malik, Y. Zafar] Abstract : Total genomic DNA from 31 available Gossypium species, three subspecies and one interspecific hybrid, were analysed to evaluate genetic diversity by RAPD, using 45 random decamer primers. A total of 579 amplified bands were observed, with 12.9 bands per primer, of which 99.8% were polymorphic. OPJ-17 produced the maximum number of fragments while the minimum number of fragments was produced with primer OPA-08. Cluster analysis by the unweighted paired group method of arithmetic means (UPGMA) showed six main clusters. Cluster 'A' consisted of two species and one subspecies of the A-genome, with a 0.78-0.92 Nei's similarity range. Cluster B, composed of all available tetraploid species and one interspecific hybrid, showed the same sister cluster. Nei's similarity ranged from 0.69 to 0.84. The B-genome formed the UPGMA sister cluster to the E-genome species. Cluster 'C' consisted of five Gossypium species of which three belong to the B-genome, with Nei's similarity values of 0.81 to 0.86. Although there was considerable disagreement at lower infra-generic ranks, particularly among the D- genome (diploid New World species) and C-genome (diploid Australian species) species. The sole F-genome species Gossypium longicalyx was resolved as a sister group to the D-genome species. Gossypium herbaceum and G. herbaceum Africanum showed the maximum Nei's similarity (0.93). Minimum similarity (0.29) was observed between Gossypium trilobum and Gossypium nelsonii. The average similarity among all studied species was 50%. The analysis revealed that the interspecific genetic relationship of several species is related to their centre of origin. As expected, most of the species have a wide genetic base range. The results also revealed the genetic relationships of the species Gossypium hirsutum to standard cultivated Gossypium barbadense, G. herbaceum and Gossypium arboreum. These results correspond well with previous reported results. The level of variation detected in closely related genotypes by RAPD analysis indicates that it may be a more efficient marker than morphological marker, isozyme and RFLP technology for the construction of genetic linkage maps. Springer-Verlag Berlin Heidelberg, 2000 Key words Gossypium species nationallicence RAPD nationallicence Phylogeny nationallicence Cluster nationallicence Diversity nationallicence Khan S. A. National Institute for Biotechnology and Genetic Engineering (NIBGE), PO Box 577, Jhang Road, Faisalabad, Pakistan e-mail: y-zafar@yahoo.com Fax: +92-41-651472, PK aut Hussain D. National Institute for Biotechnology and Genetic Engineering (NIBGE), PO Box 577, Jhang Road, Faisalabad, Pakistan e-mail: y-zafar@yahoo.com Fax: +92-41-651472, PK aut Askari E. National Institute for Biotechnology and Genetic Engineering (NIBGE), PO Box 577, Jhang Road, Faisalabad, Pakistan e-mail: y-zafar@yahoo.com Fax: +92-41-651472, PK aut McD Stewart J. Department of Agronomy, University of Arkansas, Arkansas, Fayetteville AR 72701, USA, US aut Malik K. A. National Institute for Biotechnology and Genetic Engineering (NIBGE), PO Box 577, Jhang Road, Faisalabad, Pakistan e-mail: y-zafar@yahoo.com Fax: +92-41-651472, PK aut Zafar Y. National Institute for Biotechnology and Genetic Engineering (NIBGE), PO Box 577, Jhang Road, Faisalabad, Pakistan e-mail: y-zafar@yahoo.com Fax: +92-41-651472, PK aut https://doi.org/10.1007/s001220051564 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/s001220051564 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Khan S. A. National Institute for Biotechnology and Genetic Engineering (NIBGE), PO Box 577, Jhang Road, Faisalabad, Pakistan e-mail: y-zafar@yahoo.com Fax: +92-41-651472, PK aut NATIONALLICENCE 700 1- Hussain D. National Institute for Biotechnology and Genetic Engineering (NIBGE), PO Box 577, Jhang Road, Faisalabad, Pakistan e-mail: y-zafar@yahoo.com Fax: +92-41-651472, PK aut NATIONALLICENCE 700 1- Askari E. National Institute for Biotechnology and Genetic Engineering (NIBGE), PO Box 577, Jhang Road, Faisalabad, Pakistan e-mail: y-zafar@yahoo.com Fax: +92-41-651472, PK aut NATIONALLICENCE 700 1- McD Stewart J. Department of Agronomy, University of Arkansas, Arkansas, Fayetteville AR 72701, USA, US aut NATIONALLICENCE 700 1- Malik K. A. National Institute for Biotechnology and Genetic Engineering (NIBGE), PO Box 577, Jhang Road, Faisalabad, Pakistan e-mail: y-zafar@yahoo.com Fax: +92-41-651472, PK aut NATIONALLICENCE 700 1- Zafar Y. National Institute for Biotechnology and Genetic Engineering (NIBGE), PO Box 577, Jhang Road, Faisalabad, Pakistan e-mail: y-zafar@yahoo.com Fax: +92-41-651472, PK aut Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer