caa a22 4500 475794281 CHVBK 20180406123719.0 cr unu---uuuuu 170329e20001001xx s 000 0 eng 10.1007/s001220051569 doi (NATIONALLICENCE)springer-10.1007/s001220051569 Variability in monomeric prolamins in Hordeum chilense [Elektronische Daten] [S. G. Atienza, M. J. Giménez, A. Martín, L. M. Martín] Abstract : Eighty-eight lines of the species Hordeum chilense Roem. et Schult., representative of the variability found in the latter's distribution zone, were analysed. Monomeric prolamins (protein fraction corresponding to wheat gliadins) were studied using the SDS-PAGE technique. The analysis of the different bands was performed using the programme BioImage ™ Whole Band Analyzer. Jaccard's coefficient of similarity was calculated, and the lines were grouped by cluster analysis using UPGMA. A great variability was found between the different lines studied. Forty-two different bands were identified, all of which were polymorphic. Sixty-eight different patterns of monomeric prolamins were identified within the 82 lines studied. A dendrogram was obtained from the analysis of the groups. No relationship between the distribution of the variability in the dendrogram and the geographical origin or the ecological characteristics of the species could be detected. It is concluded that H. chilense is an important pool of variability for storage proteins that could be used in cereal breeding. Springer-Verlag Berlin Heidelberg, 2000 Key words Hordeum chilense nationallicence Gliadins nationallicence Variability nationallicence SDS-PAGE nationallicence Molecular markers nationallicence Atienza S. G. Departamento de Agronomía y Mejora Genética Vegetal, Instituto de Agricultura Sostenible, Consejo Superior de Investigaciones Científicas, Apdo. 4084, E-14080 Córdoba, Spain e-mail: es2atpes@uco.es., ES aut Giménez M. J. Departamento de Genética, Escuela Técnica Superior de Ingenieros Agrónomos y de Montes, Universidad de Córdoba, Apdo. 3048, E-14080 Córdoba, Spain, ES aut Martín A. Departamento de Agronomía y Mejora Genética Vegetal, Instituto de Agricultura Sostenible, Consejo Superior de Investigaciones Científicas, Apdo. 4084, E-14080 Córdoba, Spain e-mail: es2atpes@uco.es., ES aut Martín L. M. Departamento de Genética, Escuela Técnica Superior de Ingenieros Agrónomos y de Montes, Universidad de Córdoba, Apdo. 3048, E-14080 Córdoba, Spain, ES aut https://doi.org/10.1007/s001220051569 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/s001220051569 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Atienza S. G. Departamento de Agronomía y Mejora Genética Vegetal, Instituto de Agricultura Sostenible, Consejo Superior de Investigaciones Científicas, Apdo. 4084, E-14080 Córdoba, Spain e-mail: es2atpes@uco.es., ES aut NATIONALLICENCE 700 1- Giménez M. J. Departamento de Genética, Escuela Técnica Superior de Ingenieros Agrónomos y de Montes, Universidad de Córdoba, Apdo. 3048, E-14080 Córdoba, Spain, ES aut NATIONALLICENCE 700 1- Martín A. Departamento de Agronomía y Mejora Genética Vegetal, Instituto de Agricultura Sostenible, Consejo Superior de Investigaciones Científicas, Apdo. 4084, E-14080 Córdoba, Spain e-mail: es2atpes@uco.es., ES aut NATIONALLICENCE 700 1- Martín L. M. Departamento de Genética, Escuela Técnica Superior de Ingenieros Agrónomos y de Montes, Universidad de Córdoba, Apdo. 3048, E-14080 Córdoba, Spain, ES aut Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer