caa a22 4500 475794303 CHVBK 20180406123719.0 cr unu---uuuuu 170329e20001001xx s 000 0 eng 10.1007/s001220051551 doi (NATIONALLICENCE)springer-10.1007/s001220051551 Saturation mapping of the apple scab resistance gene Vf using AFLP markers [Elektronische Daten] [M. L. Xu, S. S. Korban] Abstract : Using the amplified fragment length polymorphism (AFLP) technique combined with a ”narrow-down” bulk segregant strategy enabled us to quickly identify 15 tightly linked AFLP markers to the Vf gene that confers resistance to the apple scab disease. High-resolution mapping placed all 15 AFLP markers within an interval of 0.6 cM around the Vf region; 7 of them were found to be inseparable from the Vf gene, 1 was localized left of, and the remaining 7 located right of the Vf gene. In addition, eight previously identified RAPD markers were also mapped, but only three, including M18, AM19, and AL07, were localized within this short interval, and none co-segregated with the Vf gene. The saturation of the Vf region with AFLP markers will accelerate both marker-assisted selection and map-based cloning. The advantages of this ”narrow-down” strategy, estimation of physical distances among AFLP markers, and their potential application are also discussed. Springer-Verlag Berlin Heidelberg, 2000 Key words Apple nationallicence Scab resistance nationallicence Mapping nationallicence Vf gene nationallicence AFLP marker nationallicence Xu M. L. Department of Natural Resources and Environmental Sciences, University of Illinois, 310 Madigan Building, 1201 West Gregory Drive, Urbana, IL 61801, USA Fax: +1-217-333-8298, e-mail: s-korban@uiuc.edu, US aut Korban S. S. Department of Natural Resources and Environmental Sciences, University of Illinois, 310 Madigan Building, 1201 West Gregory Drive, Urbana, IL 61801, USA Fax: +1-217-333-8298, e-mail: s-korban@uiuc.edu, US aut https://doi.org/10.1007/s001220051551 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/s001220051551 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Xu M. L. Department of Natural Resources and Environmental Sciences, University of Illinois, 310 Madigan Building, 1201 West Gregory Drive, Urbana, IL 61801, USA Fax: +1-217-333-8298, e-mail: s-korban@uiuc.edu, US aut NATIONALLICENCE 700 1- Korban S. S. Department of Natural Resources and Environmental Sciences, University of Illinois, 310 Madigan Building, 1201 West Gregory Drive, Urbana, IL 61801, USA Fax: +1-217-333-8298, e-mail: s-korban@uiuc.edu, US aut Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer