caa a22 4500 47579432X CHVBK 20180406123719.0 cr unu---uuuuu 170329e20000901xx s 000 0 eng 10.1007/s001220051527 doi (NATIONALLICENCE)springer-10.1007/s001220051527 Mapping new EMBL-derived barley microsatellites and their use in differentiating German barley cultivars [Elektronische Daten] [K. Pillen, A. Binder, B. Kreuzkam, L. Ramsay, R. Waugh, J. Förster, J. Léon] Abstract : By searching the EMBL DNA sequence database, we were able to develop 39 new, database-derived barley microsatellites. Eighteen of these EMBL microsatellites were mapped either to the interspecific barley map Lerche×BGRC41936 (L×41), the Igri×Franka map (I×F, Graner et al. 1991), or to both maps simultaneously. In addition, all 39 EMBL microsatellites were assigned to individual barley chromosomes by PCR screening of wheat barley addition lines. Both studies verified a random distribution of the microsatellites within the barley genome. Subsequently, 22 EMBL microsatellites were used to assess the genetic similarity among a set of 28, mainly German, barley cultivars and two wild form accessions. Spring and winter cultivars could be easily differentiated using the first coordinate of a principal coordinate analysis. Whereas the group of spring barley cultivars appeared rather homogeneous, winter barley cultivars could be divided into three subgroups. Two H. v. ssp. spontaneum accessions were included in the assessment of genetic similarity. They were placed among the winter barley cultivars. Based on the assessment of the 30 barley cultivars and accessions, the polymorphism information content (PIC) of each EMBL microsatellite has been calculated. The average PIC value among the EMBL microsatellites was equal to 0.38, which ascertains the value of these microsatellites as a genetic tool in barley genome research projects. Springer-Verlag Berlin Heidelberg, 2000 Key words Barley nationallicence Microsatellites nationallicence Linkage map nationallicence Genetic similarity (GS) nationallicence Polymorphism information content (PIC) nationallicence Pillen K. Department of Crop Science and Plant Breeding, University of Bonn, Katzenburgweg 5, D-53115 Bonn, Germany e-mail: k.pillen@uni-bonn.de, DE aut Binder A. Department of Crop Science and Plant Breeding, University of Bonn, Katzenburgweg 5, D-53115 Bonn, Germany e-mail: k.pillen@uni-bonn.de, DE aut Kreuzkam B. Department of Crop Science and Plant Breeding, University of Bonn, Katzenburgweg 5, D-53115 Bonn, Germany e-mail: k.pillen@uni-bonn.de, DE aut Ramsay L. Scottish Crop Research Institute (SCRI), Invergowrie, Dundee, DD2 5DA, Scotland, UK, GB aut Waugh R. Scottish Crop Research Institute (SCRI), Invergowrie, Dundee, DD2 5DA, Scotland, UK, GB aut Förster J. Saaten-Union Resistenzlabor GmbH, Hovedisser Str. 92, D-33818 Leopoldshöhe, Germany, DE aut Léon J. Department of Crop Science and Plant Breeding, University of Bonn, Katzenburgweg 5, D-53115 Bonn, Germany e-mail: k.pillen@uni-bonn.de, DE aut https://doi.org/10.1007/s001220051527 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/s001220051527 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Pillen K. Department of Crop Science and Plant Breeding, University of Bonn, Katzenburgweg 5, D-53115 Bonn, Germany e-mail: k.pillen@uni-bonn.de, DE aut NATIONALLICENCE 700 1- Binder A. Department of Crop Science and Plant Breeding, University of Bonn, Katzenburgweg 5, D-53115 Bonn, Germany e-mail: k.pillen@uni-bonn.de, DE aut NATIONALLICENCE 700 1- Kreuzkam B. Department of Crop Science and Plant Breeding, University of Bonn, Katzenburgweg 5, D-53115 Bonn, Germany e-mail: k.pillen@uni-bonn.de, DE aut NATIONALLICENCE 700 1- Ramsay L. Scottish Crop Research Institute (SCRI), Invergowrie, Dundee, DD2 5DA, Scotland, UK, GB aut NATIONALLICENCE 700 1- Waugh R. Scottish Crop Research Institute (SCRI), Invergowrie, Dundee, DD2 5DA, Scotland, UK, GB aut NATIONALLICENCE 700 1- Förster J. Saaten-Union Resistenzlabor GmbH, Hovedisser Str. 92, D-33818 Leopoldshöhe, Germany, DE aut NATIONALLICENCE 700 1- Léon J. Department of Crop Science and Plant Breeding, University of Bonn, Katzenburgweg 5, D-53115 Bonn, Germany e-mail: k.pillen@uni-bonn.de, DE aut Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer