caa a22 4500 475794346 CHVBK 20180406123719.0 cr unu---uuuuu 170329e20000901xx s 000 0 eng 10.1007/s001220051522 doi (NATIONALLICENCE)springer-10.1007/s001220051522 Genetic control of early events in protoplast division and regeneration pathways in sunflower [Elektronische Daten] [E. Flores Berrios, L. Gentzbittel, L. Mokrani, G. Alibert, A. Sarrafi] Abstract : Experiments were conducted to identify the genetic factors controlling protoplast division and to determine eventual relations between genetic factors involving organogenesis, somatic embryogenesis and protoplast division in sunflower. The present study involved protoplast culture and two traits: total division per 100 protoplasts (TOTD) and asymmetric division per 100 protoplasts (ASYD) were scored in 52 recombinant inbred lines (RILs) from a cross between PAC-2 and RHA-266. Asymmetric division is an early event in the formation of embryoids from protoplasts. Analysis of variance indicated the existence of highly significant differences among parental genotypes and their RILs. Heritability for the two protoplast division parameters (TOTD and ASYD) was high (0.87 and 0.89, respectively) and genetic gain expressed as percentage of the best parent for 10% of the selected RILs was significant. Twelve putative loci associated with total division per 100 protoplasts were identified. Eleven QTLs were also detected for asymmetric division per 100 protoplasts. The QTLs present high significant LOD scores and sum to a high percentage of phenotypic variance. The percentage of phenotypic variation explained by each QTL ranged from 2% to 24%. Some segments of the linkage groups I, XV and XVII are likely to contain genes important for organogenesis, somatic embryogenesis and protoplast division, as clustering of QTLs for these characters were described. The QTLs identified in these three linkage groups should be involved in cell division and in early events associated with cell differenciation. Springer-Verlag Berlin Heidelberg, 2000 Key words Sunflower nationallicence Helianthus annuus L nationallicence AFLP nationallicence QTL nationallicence Protoplast nationallicence In vitro regeneration nationallicence Flores Berrios E. Department of Biotechnology and Plant Breeding, Pôle de Biotechnologie Végétale, BAP, INP-ENSAT 18 Chemin de Borde Rouge, BP 107, 31326 Castanet France e-mail: sarrafi@ensat.fr Tel: +33 (0)5 62 19 35 80, Fax: +33 (0)5 62 19 35 81, FR aut Gentzbittel L. Department of Biotechnology and Plant Breeding, Pôle de Biotechnologie Végétale, BAP, INP-ENSAT 18 Chemin de Borde Rouge, BP 107, 31326 Castanet France e-mail: sarrafi@ensat.fr Tel: +33 (0)5 62 19 35 80, Fax: +33 (0)5 62 19 35 81, FR aut Mokrani L. Department of Biotechnology and Plant Breeding, Pôle de Biotechnologie Végétale, BAP, INP-ENSAT 18 Chemin de Borde Rouge, BP 107, 31326 Castanet France e-mail: sarrafi@ensat.fr Tel: +33 (0)5 62 19 35 80, Fax: +33 (0)5 62 19 35 81, FR aut Alibert G. Department of Biotechnology and Plant Breeding, Pôle de Biotechnologie Végétale, BAP, INP-ENSAT 18 Chemin de Borde Rouge, BP 107, 31326 Castanet France e-mail: sarrafi@ensat.fr Tel: +33 (0)5 62 19 35 80, Fax: +33 (0)5 62 19 35 81, FR aut Sarrafi A. Department of Biotechnology and Plant Breeding, Pôle de Biotechnologie Végétale, BAP, INP-ENSAT 18 Chemin de Borde Rouge, BP 107, 31326 Castanet France e-mail: sarrafi@ensat.fr Tel: +33 (0)5 62 19 35 80, Fax: +33 (0)5 62 19 35 81, FR aut https://doi.org/10.1007/s001220051522 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/s001220051522 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Flores Berrios E. Department of Biotechnology and Plant Breeding, Pôle de Biotechnologie Végétale, BAP, INP-ENSAT 18 Chemin de Borde Rouge, BP 107, 31326 Castanet France e-mail: sarrafi@ensat.fr Tel: +33 (0)5 62 19 35 80, Fax: +33 (0)5 62 19 35 81, FR aut NATIONALLICENCE 700 1- Gentzbittel L. Department of Biotechnology and Plant Breeding, Pôle de Biotechnologie Végétale, BAP, INP-ENSAT 18 Chemin de Borde Rouge, BP 107, 31326 Castanet France e-mail: sarrafi@ensat.fr Tel: +33 (0)5 62 19 35 80, Fax: +33 (0)5 62 19 35 81, FR aut NATIONALLICENCE 700 1- Mokrani L. Department of Biotechnology and Plant Breeding, Pôle de Biotechnologie Végétale, BAP, INP-ENSAT 18 Chemin de Borde Rouge, BP 107, 31326 Castanet France e-mail: sarrafi@ensat.fr Tel: +33 (0)5 62 19 35 80, Fax: +33 (0)5 62 19 35 81, FR aut NATIONALLICENCE 700 1- Alibert G. Department of Biotechnology and Plant Breeding, Pôle de Biotechnologie Végétale, BAP, INP-ENSAT 18 Chemin de Borde Rouge, BP 107, 31326 Castanet France e-mail: sarrafi@ensat.fr Tel: +33 (0)5 62 19 35 80, Fax: +33 (0)5 62 19 35 81, FR aut NATIONALLICENCE 700 1- Sarrafi A. Department of Biotechnology and Plant Breeding, Pôle de Biotechnologie Végétale, BAP, INP-ENSAT 18 Chemin de Borde Rouge, BP 107, 31326 Castanet France e-mail: sarrafi@ensat.fr Tel: +33 (0)5 62 19 35 80, Fax: +33 (0)5 62 19 35 81, FR aut Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer