caa a22 4500 475794419 CHVBK 20180406123719.0 cr unu---uuuuu 170329e20000901xx s 000 0 eng 10.1007/s001220051518 doi (NATIONALLICENCE)springer-10.1007/s001220051518 Unreduced diploid nuclei in Cupressus dupreziana A. Camus pollen [Elektronische Daten] [C. Pichot, M. El Maâtaoui] Abstract : Size and DNA content of pollen of Cupressus dupreziana A. Camus, a highly endangered Mediterranean conifer, were assessed by cytomorphological observations and flow cytometric analyses and then compared to C. sempervirens L. pollen. Mature C. dupreziana pollen was composed of two uninucleated types of pollen grains differing in size. Around 35% of the grains exhibited a size similar to C. sempervirens pollen, while 65% exhibited a larger diameter. However only one peak of fluorescence was detected by flow cytometry. DNA content of C. dupreziana pollen was twice the DNA content of C. sempervirens pollen. Comparison of DNA contents of mature and germinating pollen revealed that mature pollen of both species were arrested in the G2 phase. Comparison with the DNA content of somatic tissue (2C) provided evidence for the production of unreduced pollen in C. dupreziana. This unexpected feature in gymnosperms is discussed in terms of reproductive strategy of this species. Springer-Verlag Berlin Heidelberg, 2000 Key words 2n pollen nationallicence Cupressus dupreziana nationallicence Gymnosperms nationallicence Flow cytometry nationallicence Reproduction nationallicence Pichot C. INRA, Unité de Recherches Forestières Méditerranéennes, Equipe de Génétique Av. Vivaldi, 84000 Avignon, France e-mail: pichot@avignon.inra.fr Fax: +33(0)4 90 13 59 59, FR aut El Maâtaoui M. Université d'Avignon, Faculté des Sciences, Département de Biologie, 33 rue L. Pasteur, 84000 Avignon, France, FR aut https://doi.org/10.1007/s001220051518 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/s001220051518 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Pichot C. INRA, Unité de Recherches Forestières Méditerranéennes, Equipe de Génétique Av. Vivaldi, 84000 Avignon, France e-mail: pichot@avignon.inra.fr Fax: +33(0)4 90 13 59 59, FR aut NATIONALLICENCE 700 1- El Maâtaoui M. Université d'Avignon, Faculté des Sciences, Département de Biologie, 33 rue L. Pasteur, 84000 Avignon, France, FR aut Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer